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You are here:Home » Antibodies » Abs to Enzymes, Phosphatase » Anti -DARPP32 (Dopamine and cAMP-regulated Phosphoprotein, Protein Phosphatase 1 Regulatory Subunit 1B, PP1R1B)

Anti -DARPP32 (Dopamine and cAMP-regulated Phosphoprotein, Protein Phosphatase 1
Regulatory Subunit 1B, PP1R1B)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IP IH IF
DARPP-32 (dopamine and cyclic AMP-regulated phosphoprotein, relative molecular mass 32,000) is a cytosolic protein highly enriched in medium-sized spiny neurons of the neostriatum (1). It is a bifunctional signaling molecule that controls serine/threonine kinase and serine/threonine phosphatase activity (2). Dopamine stimulates phosphorylation of DARPP-32 through D1 receptors and activation of PKA. PKA phosphorylation of DARPP-32 at threonine 34 converts it into an inhibitor of protein phosphatase 1 (1). DARPP-32 is converted into an inhibitor of PKA when phosphorylated at threonine 75 by cyclin-dependent kinase 5 (CDK5) (2). Mice containing a targeted deletion of the DARPP-32 gene exhibit an altered biochemical, electrophysiological and behavioral phenotype (3).
Catalog #D1075-07
ApplicationsSuitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunofluorescence. Other applications have not been tested.
Recommended DilutionsWestern Blot: 1:1000
Immunoprecipitation: 1:50
Immunohistochemistry (Paraffin): 1:200 using citrate/TBST for antigen retrieval.
Immunofluorescence (Frozen): 1:400
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG
Clone No7H17
HostRabbit
ConcentrationAs reported
FormSupplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
PuritySupernatant
ImmunogenSynthetic peptide corresponding to residues surrounding Glu160 of human DARPP-32 (KLH). Species Sequence Homology: human.
SpecificityReccognizes endogenous levels of total DARPP-32 protein. Species Crossreactivity: mouse, rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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