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You are here:Home » Antibodies » Abs to Oncoproteins » Anti -DBC1 (Protein KIAA1967, Deleted in Breast Cancer Gene 1 Protein, DBC-1, DBC.1, p30 DBC, KIAA1967)

Anti -DBC1 (Protein KIAA1967, Deleted in Breast Cancer Gene 1 Protein, DBC-1, DBC.1, p30
DBC, KIAA1967)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP IF
Deleted in breast cancer gene 1 protein (DBC1) was originally identified by its localization to a region of chromosome 8p21 that is homozygously deleted in breast cancer (1). DBC1 is a large, nuclear protein with multiple functions in cell survival. It binds directly to the estrogen receptor a (ERa) hormone-binding domain in a ligand-independent manner and may be a key determinant of ligand-independent ERa expression and survival in human breast cancer cells (2). DBC1 can promote p53-mediated apoptosis by binding to and inhibiting the deacetylase activity of SirT1, resulting in increased p53 acetylation levels and activity (3). DBC1 may be an important regulator of heterochromatin formation as it binds SUV39H1 and inhibits its histone methyltransferase activity (4). Caspase-dependent processing activates the pro-apoptotic activity of DBC1 during Tumor Necrosis Factor-a (TNF-a)-mediated cell death signaling (5). This processing of DBC1 in response to TNF-a is an early event in the onset of apoptosis and results in relocalization of DBC1 to the cytoplasm. Over-expression of the processed, cytoplasmic form of DBC1 results in mitochondrial clustering and matrix condensation and sensitizes cells to TNF-a-mediated apoptosis.
Catalog #D1171-01F
ApplicationsSuitable for use in Western Blot, Immunofluorescence and Immunoprecipitation. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000
Immunoprecipitation: 1:50
Immunofluorescence: 1:100
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptide corresponding to residues near the amino terminus of human DBC1.
SpecificityRecognizes endogenous levels of total human DBC1. Species Crossreactivity: Equine, monkey, mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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