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You are here:Home » Antibodies » Abs to Retinoblastoma Proteins » Anti -DDX1 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 1, DBP-RB, DEAD Box Protein-Retinoblastoma DDX-1)

Anti -DDX1 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 1, DBP-RB, DEAD Box
Protein-Retinoblastoma DDX-1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
DEAD box proteins, a family of putative RNA helicases, are characterized by eight conserved amino acid motifs that are arranged in a core region as found in the prototypical member of the family, eIF-4A. The family's name is derived from the amino acid sequence Asp-Glu-Ala-Asp (DEAD) that is located within the ATP hydrolysis motif. DEAD box proteins have been implicated in translation initiation and RNA splicing, degradation, and stability. The DEAD box protein, DDX1, was identified in retinoblastoma cell lines, where it is coamplified with MYCN, a member of the MYC transcription factor family. DDX1 is expressed at elevated levels in fetal tissues of neural origin (retina and brain). It is both cytoplasmic and nuclear in DDX1-amplified neuroblastoma and retinoblastoma cell lines. However, it localizes specifically to the nucleus in non-amplified cell lines. Thus, DDX1 is thought to be involved in nuclear RNA metabolism. In addition, the decrease in mean disease-free survival of neuroblastoma patients exhibiting coamplification of DDX1 and MYCN as compared to those exhibiting MYCN amplification indicates a possible role for DDX1 in cancer formation in neural tissues.
Catalog #D1660-17
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a liquid in TBS, pH 7.2, 0.1% BSA, 0.1% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenThe epitope maps to a region between residues 500 and 550 of human DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 1 using the numbering given in entry AAH12739.1 (GeneID 1653).
SpecificityThis antibody is specific for DDX1 protein. Species Crossreactivity: Human. Reactivity with mouse DDX1 is expected owing to identity of sequence between this ortholog and human DDX1 at the site to which the epitope maps.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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