| Catalog # | D1660-22 |
| Description | DDX3 contains all of the motifs of the DEAD-box family of RNA helicases, including the Asp-Glu-Ala-Asp sequence that gives the protein family its name and distinguishes it |
| from other RNA helicases (1,2). DDX3 is localized to the |
| X chromosome and has a highly conserved functional |
| homolog (DBY) on the Y chromosome (3). DDX3 is |
| thought to be involved in RNA splicing, RNA transport, and |
| translation initiation (4). It has also been shown to be |
| involved in cell growth control and is deregulated in |
| hepatitis virus-associated hepatocellular carcinoma (5). |
| Recent experiments suppressing DDX3 expression |
| blocked HIV-1 RNA export from the nucleus, suggesting |
| that DDX3 functions as a shuttling protein that transports |
| the HIV-1 protein Rev and its cofactor CRM1 from the |
| nucleus to the cytoplasm (6). |
| |
| Applications: |
| Suitable for use in Western Blot. Other applications not tested. |
| Recommended Dilution | Western Blot: 0.5-1ug/ml. |
| Optimal dilutions to be determined by the researcher. |
| Postive control | HepG2 cell lysate |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Human |
| Concentration | As reported |
| Form | Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide. |
| Purity | Purified by immunoaffinity chromatography. |
| Immunogen | Synthetic peptide 16 aa, near the amino terminus of human DDX3 (Genbank accession No. AAC34298). |
| Specificity | Recognizes human DDX3, |
|
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
