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You are here:Home » Antibodies » Antibodies-DEAD Box Proteins » Anti -DDX3, NT (DEAD Box Protein 3 X-chromosomal, DBX, Helicase-Like Protein 2, HLP2)

Anti -DDX3, NT (DEAD Box Protein 3 X-chromosomal, DBX, Helicase-Like Protein 2, HLP2)


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Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Catalog #D1660-22
DescriptionDDX3 contains all of the motifs of the DEAD-box family of RNA helicases, including the Asp-Glu-Ala-Asp sequence that gives the protein family its name and distinguishes it
from other RNA helicases (1,2). DDX3 is localized to the
X chromosome and has a highly conserved functional
homolog (DBY) on the Y chromosome (3). DDX3 is
thought to be involved in RNA splicing, RNA transport, and
translation initiation (4). It has also been shown to be
involved in cell growth control and is deregulated in
hepatitis virus-associated hepatocellular carcinoma (5).
Recent experiments suppressing DDX3 expression
blocked HIV-1 RNA export from the nucleus, suggesting
that DDX3 functions as a shuttling protein that transports
the HIV-1 protein Rev and its cofactor CRM1 from the
nucleus to the cytoplasm (6).
Suitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 0.5-1ug/ml.
Optimal dilutions to be determined by the researcher.
Postive controlHepG2 cell lysate
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
ConcentrationAs reported
FormSupplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide 16 aa, near the amino terminus of human DDX3 (Genbank accession No. AAC34298).
SpecificityRecognizes human DDX3,
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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