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You are here:Home » Antibodies » Abs to DEAD Box Proteins » Anti -DDX3 (DEAD Box Protein 3 X-chromosomal, DBX, Helicase-Like Protein 2, HLP2)

Anti -DDX3 (DEAD Box Protein 3 X-chromosomal, DBX, Helicase-Like Protein 2, HLP2)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP IH IC FC
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which interacts specifically with hepatitis C virus core protein resulting in a change in intracellular location. This gene has a homolog located in the nonrecombining region of the Y chromosome. The protein sequence is 91% identical between this gene and the Y-linked homolog. Alternative splicing of this gene generates 2 transcript variants, which are divergent in the 3' UTR.
Catalog #D1660-22D
ApplicationsSuitable for use in Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionsWestern Blot: 0.04-0.1ug/ml
Immunoprecipitation: 2-5ug/mg lysate
Optimal dilutions to be determined by the researcher.
Positive ControlHeLa cells
Storage and StabilityMay be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in Tris-citrate/phosphate, pH 7.5, 0.09% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenThe epitope that this antibody recognizes maps to a region between residues 600 and the C-terminus (residue 661) of human DEAD (Asp-Glu-Ala-Asp) box polypeptide 3 using the numbering given in entry NP_001347.2 (GeneID 1654).
SpecificityRecognizes human DDX3.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesDDX 3, DDX-3, DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 3, DEAD Box Polypeptide, X-linked, CAP-Rf


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