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Abs to DEAD Box Proteins
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Anti -DDX4 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 4, DEAD Box Protein 4, MGC111074, Probable ATP-dependent RNA Helicase DDX4, Vasa Homolog, VASA)
Anti -DDX4 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 4, DEAD Box Protein 4, MGC111074, Probable ATP-dependent RNA Helicase DDX4, Vasa Homolog, VASA)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Purified |
B IP |
|
| DDX4 is an ATP-dependent DEAD-box RNA helicase found in the chromatoid body of the germ cells (1). This enzyme is specific to the germ cells and is necessary for germ cell development (2). Mouse DDX4 was shown to interact with Dicer suggesting its role in the icroRNA-mediated | | | RNA silencing (1). | | | Catalog # | D1660-27 | | Applications | Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. | | Recommended Dilution | Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. | | Immunoprecipitation: 1:50 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | 11C1025 | | Host | Mouse | | Source | Human | | Concentration | Not determined | | Form | Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, less than 0.02% sodium azide, 50% glycerol. | | Purity | Purified | | Immunogen | Purified recombinant fragment of human DDX4. | | Specificity | Recognizes endogenous levels of total DDX4. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. | | Alternate names | EC=3.6.4.13 |
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