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Abs to DEAD Box Proteins
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Anti -DDX5 (DEAD (Asp-Glu-Ala-Asp) Box 5, DKFZp686J01190, G17P1, HLR1, HUMP68, p68)
Anti -DDX5 (DEAD (Asp-Glu-Ala-Asp) Box 5, DKFZp686J01190, G17P1, HLR1, HUMP68, p68)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
B |
|
| DDX5, also known as p68 RNA helicase, is a 614aa, 68kD member of the DEAD box family of RNA helicases that contain the conserved Asp-Glu-Ala-Asp (DEAD) motif. DDX5 influences RNA transport, transcription, ribosome assembly, spliceosome function, RNA degradation, and the initiation of translation. DDX5 shares 87% aa identity with DDX-17 within the central region and the two are often expressed together. The C-terminal region used as an immunogen shares 96% and 99% aa identity with mouse and rat DDX5, respectively, but lies mainly outside the DDX-17 homology region. | | | Catalog # | D1660-40F | | Applications | Suitable for use in Western Blot. Other applications not tested. | | Recommended Dilution | Western Blot: 0.1ug/ml | | Optimal dilutions to be determined by the researcher. | | Hybridoma | Myeloma cells with B cells from Balb/c mice. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG2a | | Clone No | 11D47 | | Host | Mouse | | Source | Human | | Concentration | Not determined | | Form | Supplied as a lyophilized powder in PBS with 5% trehalose. Reconstitute with sterile PBS. | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | Recombinant corresponding to aa448-614 from human DDX5 expressed in E. coli (P17844). | | Specificity | Recognizes human and mouse DDX5. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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