Anti -DDX5 (Probable ATP-dependent RNA Helicase DDX5, DEAD Box Protein 5, RNA Helicase
p68, G17P1, HELR, HLR1)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Goat |
Affinity Purified |
E B IH |
|
| DDX5, also known as p68 RNA helicase, is a 614aa, 68kD member of the DEAD box family of RNA helicases that contain the conserved Asp-Glu-Ala-Asp (DEAD) motif. DDX5 influences RNA transport, transcription, ribosome assembly, spliceosome function, RNA degradation, and the initiation of translation. DDX5 shares 87% aa identity with DDX-17 within the central region and the two are often expressed together. The C-terminal region used as an immunogen shares 96% and 99% aa identity with mouse and rat DDX5, respectively, but lies mainly outside the DDX-17 homology region. | | | Catalog # | D1660-40H | | Applications | Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. | | Recommended Dilution | ELISA: 1:64,000 | | Western Blot: 1:64,000 & 0.11-0.3ug/ml | | Immunohistochemistry (Formalin fixed paraffin embedded): 2.5ug/ml | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Polyclonal | | Host | Goat | | Source | Human | | Concentration | ~0.5mg/ml | | Form | Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Synthetic peptide corresponding to C-PMIGYPMPTGYSQ from C-terminus of human DDX5. | | Specificity | Recognizes human DDX5. Species sequence homology: Canine, mouse, rat. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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