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You are here:Home » Antibodies » Antibodies-DEAD Box Proteins » Anti -DDX5 (Probable ATP-dependent RNA Helicase DDX5, DEAD Box Protein 5, RNA Helicase p68, G17P1, HELR, HLR1)

Anti -DDX5 (Probable ATP-dependent RNA Helicase DDX5, DEAD Box Protein 5, RNA Helicase
p68, G17P1, HELR, HLR1)


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Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH
DDX5, also known as p68 RNA helicase, is a 614aa, 68kD member of the DEAD box family of RNA helicases that contain the conserved Asp-Glu-Ala-Asp (DEAD) motif. DDX5 influences RNA transport, transcription, ribosome assembly, spliceosome function, RNA degradation, and the initiation of translation. DDX5 shares 87% aa identity with DDX-17 within the central region and the two are often expressed together. The C-terminal region used as an immunogen shares 96% and 99% aa identity with mouse and rat DDX5, respectively, but lies mainly outside the DDX-17 homology region.
Catalog #D1660-40J
ApplicationsSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionELISA: 1:64,000
Western Blot: 0.11-0.3ug/ml, observed in human epidermoid carcinoma A431 lysates on ~70kD bands
Immunohistochemistry (Paraffin): 2-5ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
FormSupplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to C-PMIGYPMPTGYSQ, from human DDX5, at C-terminal (NP_004387.1).
SpecificityRecognizes human DDX5. Species sequence homology: Canine, mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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