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You are here:Home » Antibodies » Abs to DEAD Box Proteins » Anti -DDX20 (DDX-20, DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 20, DEAD-box Protein DP103, SMN-interacting Protein, DKFZp434H052, Gemin 3)

Anti -DDX20 (DDX-20, DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 20, DEAD-box Protein DP103,
SMN-interacting Protein, DKFZp434H052, Gemin 3)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP
The survival of motor neurons (SMN) gene is the disease gene of spinal muscular atrophy (SMA), a common motor neuron degenerative disease. The SMN protein is part of a complex containing several proteins, of which one, SIP1 (SMN interacting protein 1), has been characterized so far. The SMN complex is found in both the cytoplasm and in the nucleus, where it is concentrated in bodies called gems. In the cytoplasm, SMN and SIP1 interact with the Sm core proteins of spliceosomal small nuclear ribonucleoproteins (snRNPs), and they play a critical role in snRNP assembly. In the nucleus, SMN is required for pre-mRNA splicing, likely by serving in the regeneration of snRNPs. A DEAD box putative RNA helicase, named Gemin 3 which is another component of the SMN complex, has been identified. Gemin 3 interacts directly with SMN, as well as with SmB, SmD2 and SmD3. Immunolocalization studies using mAbs to Gemin 3 show that it co-localizes with SMN in gems. Gemin 3 binds SMN via its unique COOH-terminal domain, and SMN mutations found in some SMA patients strongly reduce this interaction. The presence of a DEAD box motif in Gemin 3 suggests that it may provide the catalytic activity that plays a critical role in the function of the SMN complex on RNPs.
Catalog #D1661-02A
ApplicationsSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a liquid in TBS, pH 7.2, 0.1% BSA, 0.1% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenThe epitope recognized maps to a region between residues 675 and 725 of human DEAD (Asp-Glu-Ala-Asp) Box 20 using the numbering given in entry NP_009135.3 (GeneID 11218).
SpecificitySpecies Crossreactivity: This antibody crossreacts with human. Other species have not been tested.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesDP103


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