Anti -DDX54 (DDX-54, DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 54, DEAD Box Protein 54, DP97,
DP-97, DEAD Box Helicase 97kD, MGC2835) (Azide Free)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
E B IH IF |
|
| This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The nucleolar protein encoded by this gene interacts in a hormone-dependent manner with nuclear receptors, and represses their transcriptional activity. Alternative splice variants that encode different isoforms have been found for this gene. | | | Catalog # | D1661-54B | | Applications | Suitable for use in ELISA, Immunofluorescence, Immunohistochemistry and Western Blot. Other applications not tested. | | Recommended Dilution | Immunohistochemistry (paraffin): 1.5ug/ml | | ELISA: 1ug/ml-3ng/ml | | Western Blot: 1:500-1:1000 | | Optimal dilutions to be determined by the researcher. | | Hybridoma | Sp2/0 myeloma cells with spleen cells from Balb/c mice. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG2a | | Clone No | 10B2683 | | Host | Mouse | | Source | Human | | Concentration | ~0.5mg/ml | | Form | Supplied as a liquid in PBS, pH 7.2. No preservative added (Azide free). | | Purity | Purified by Protein A affinity chromatography. | | Immunogen | Recombinant protein corresponding to a portion of amino acids within aa778-882 of human DDX54. | | Specificity | Recognizes human DDX54. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. | | Alternate names | 2410015A15Rik, ATP Dependent RNA Helicase DDX54 |
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