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Specifications

When supplemented with appropriate substrates, used to differentiate gram negative enteric bacteria.
Catalog #	D1830
	Moeller first described the use of an amino acid decarboxylase test to distinguish between various microorganisms. He further noted that the production of lysine, arginine, ornithine and glutamic acid decarboxylase helped to differentiate between gram-negative enteric bacteria. Ewing et al. concluded, after investigating the decarboxylase activity of 2937 cultures of the Enterobacteriaceae, that the Moeller formulation should be considered the standard medium for studying decarboxylate reactions. Bacteria which produce decarboxylase will produce an alkaline reaction (purple or violet). Decarboxylase negative bacteria will give an acidic reaction (yellow) due to the fermentation of dextrose. To insure proper reactions overlay medium with sterile mineral oil.
Microbiological Analysis	(Lys/Arg/Orn)
Enterobacter aerogenes ATCC 13048	Growth ( + / - / + )
Escherichia coli ATCC 25922	Growth ( + / - / + )
klebsiella pneumoniae ATCC 13833	Growth ( + / - / -)
Appearance
Light beige, homogeneous, free flowing powder.
pH (25°C)
6.0±0.2
Storage
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.