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You are here:Home » Molecular Biology » Lectin Other » Dectin 2, Recombinant, Human (CLEC6A, CLECSF10, NKCL)

Dectin 2, Recombinant, Human (CLEC6A, CLECSF10, NKCL)

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Specifications

Dectin-2, also known as CLEC6A, CLECSF10, and NKCL, belongs to the C-type lectin family of transmembrane immune regulatory glycoproteins. Dectin-2, plus CLEC4A-E constitute a subgroup of molecules that exhibit approximately 40% aa sequence identity in their extracellular domains (ECD), and have a conserved cysteine spacing in their carbohydrate recognition domains (CRD) (1, 2). Mature human Dectin-2 is a type II transmembrane protein with a short cytoplasmic tail, a transmembrane segment, and a 168 aa ECD with a stalk region and one CRD (3, 4). Within the ECD, human Dectin-2 shares 71% and 75% aa sequence identity with bovine and mouse Dectin-2, respectively. An alternately spliced isoform has a deletion of portions of the transmembrane and cytoplasmic regions (5). Full length Dectin-2 is a 27 kD molecule that is expressed on monocytes, tissue macrophages, and activated CD4+ T cells (4-6). The CRD of Dectin-2 contains an EPN motif which is characteristic of calcium-dependent mannose-binding lectins. Dectin-2 selectively interacts with high mannose structures in the Man9GlcNAc2 configuration (7). It mediates the recognition of a variety of microorganisms, particularly the filamentous forms of yeast and fungii (7, 8). The short cytoplasmic tail does not contain signaling motifs but mediates association with the ITAM-containing Fc receptor subunit on macrophages (8). Ligation of Dectin-2 induces tyrosine phosphorylation of the subunit, activation of NF B, and enhanced release of TNF- and IL-1ra (8). Macrophage Dectin-2 is upregulated in vivo by inflammatory stimuli and UV-B irradiation (5, 6, 9). Dectin-2 is known to participate in UV-induced immunosuppression by interacting with CD4+ CD25+ regulatory T cells, which then induce dendritic cells to release IL-4, IL-10, and TGF- (10).
Catalog #D1876-50
SourceHuman CD33 Signal Peptide (Met 1-Ala 16), HHHHHH, Human Dectin-2 (Thr 46-Leu 209). A DNA sequence encoding the signal peptide from human CD33 was fused to the N-terminal 6X histidine-tagged extracellular domain of human Dectin-2 (Thr 46-Leu 209) (Accession # NP_001007034; Q6EIG7). The chimeric protein was expressed in a mouse myeloma cell line, NS0.
Molecular MassBased on N-terminal amino acid sequencing, the recombinant human Dectin-2 begins with the histidine tag. It has a predicted molecular mass of approximately 19.7 kD. As a result of glycosylation, the recombinant human Dectin-2 migrates as an approximately 23 kD protein in SDS-PAGE under reducing conditions.
Endotoxin Level< 1.0 EU per 1 g of the protein as determined by the LAL method.
ActivityMeasured by its ability to bind biotinylated -D-Mannose-PAA in a functional ELISA.
ReconstitutionIt is recommended that sterile PBS be added to the vial to prepare a working stock solution of no less than 100ug/ml. The carrier-free protein should be used immediately upon reconstitution to avoid losses in activity due to non-specific binding to the inside surface of the vial. For long term storage as a dilute solution, a carrier protein (e.g. 0.1% HSA or BSA) should be added to the vial.
Storage and StabilityLyophilized samples are stable for up to twelve months at -20°C. Upon reconstitution, this protein, in the presence of a carrier protein, can be stored under sterile conditions at 2-8°C for one month or at -20°C in a manual defrost freezer for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
SourceMouse myeloma cell line, NS0
Purity90%, as determined by SDS-PAGE and visualized by silver stain.
ConcentrationAs reported
FormSupplied as a lyophilized powder in PBS.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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