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You are here:Home » Molecular Biology » Lectin Other » Dectin 2a, Recombinant, Mouse (CLEC6A, CLECSF10, NKCL)

Dectin 2a, Recombinant, Mouse (CLEC6A, CLECSF10, NKCL)

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Specifications

Dectin-2 , also known as CLEC6A, CLECSF10, and NKCL, belongs to the C-type lectin family of transmembrane immune regulatory glycoproteins. Dectin-2 , CLEC4A/DCIR, CLEC4B/DCAR, CLEC4C/DLEC, CLEC4D/MCL, and CLEC4E/mincle constitute a subgroup of these molecules that exhibit approximately 40% aa sequence identity in their extracellular domains (ECD) and have a conserved cysteine spacing in their carbohydrate recognition domains (CRD). 1,2 Mature mouse dectin-2 consists of a short cytoplasmic tail, a transmembrane segment, and an ECD with a stalk region and one CRD. 3,4 Alternate splicing leads to partial deletion of the transmembrane segment and stalk ( isoform) or a portion of the CRD ( isoform). 4 The full length dectin-2 isoform is a 27 kD molecule that is primarily expressed on the surface of tissue macrophages and their precursors. 3-6 The CRD of dectin-2 contains an EPN motif which is characteristic of calcium-dependent mannose-binding lectins. Dectin-2 selectively interacts with high mannose structures in the Man9GlcNAc2 configuration. 7 It mediates the recognition of a variety of microorganisms, particularly the filamentous forms of yeast and fungii. 7,8 The short cytoplasmic tail does not contain signaling motifs but mediates association with the ITAM-containing Fc receptor subunit in macrophages. 8 Ligation of dectin-2 induces tyrosine phosphorylation of the subunit, activation of NF B, and enhanced release of TNF- and IL-1ra. 8 Macrophage dectin-2 is upregulated in vivo by inflammatory stimuli and UV-B irradiation. 6 It mediates the breaking of UV-induced tolerance by interacting with CD4+ CD25+ regulatory T cells which then induce dendritic cells to release IL-4, IL-10, and TGF- . 9 Within the ECD, mouse dectin-2 shares 71% aa sequence identity with human and bovine dectin-2.
Catalog #D1876-51
SourceHuman CD33 signal peptide (Met 1-Ala 16), HHHHHHHA, Mouse Dectin-2 (Phe43-Leu209). A DNA sequence encoding the N-terminal polyhistidine tagged extracellular domain of mouse dectin-2 (Phe 43-Leu 209; Accession # AAF67177) (Ariizumi, K., et al., 2000 J. Biol. Chem., 275: 11957-63) was fused to the signal peptide of human CD33 at the N-terminus. The protein was expressed in a mouse myeloma cell line, NS0.
Molecular MassThe recombinant mature polyhistidine tagged mouse dectin-2 , generated by the removal of the signal peptide region, is a monomeric protein. Based on N-terminal sequencing, the mature recombinant mouse dectin-2 begins with the histidine tag and has a calculated molecular mass of 20.6 kD. As a result of glycosylation, the recombinant protein migrates as an approximately 24 kD protein in SDS- PAGE under reducing conditions.
Endotoxin Level< 1.0 EU per 1 g of the protein as determined by the LAL method.
ActivityMeasured by its ability to bind biotinylated -D-Mannose-PAA in a functional ELISA.
ReconstitutionIt is recommended that sterile PBS be added to the vial to prepare a working stock solution of no less than 100ug/ml. The carrier-free protein should be used immediately upon reconstitution to avoid losses in activity due to non-specific binding to the inside surface of the vial. For long term storage as a dilute solution, a carrier protein (e.g. 0.1% HSA or BSA) should be added to the vial.
Storage and StabilityLyophilized samples are stable for up to twelve months at -20°C. Upon reconstitution, this protein, in the presence of a carrier protein, can be stored under sterile conditions at 2-8°C for one month or at -20°C in a manual defrost freezer for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
SourceMouse myeloma cell line, NS0
Purity95%, as determined by SDS-PAGE and visualized by silver stain.
ConcentrationAs reported
FormSupplied as a lyophilized powder in PBS.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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