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You are here:Home » Antibodies » Abs to Defensin » Anti -Defensin

Anti -Defensin

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified IH FC
Defensins are a group of cyclic peptides containing 29-35 amino acids, which comprise approximately 30% of the total protein content of neutrophil azurophilic granules. Various functions have been reported for the defensins, including antibacterial and antifungal activity and chemotactic activity for monocytes. 3H2072 recognizes alpha defensins (human neutrophil peptides). Binding to defensin 4 has not been tested.
Catalog #D1950
ApplicationsSuitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunohistochemistry: (frozen and paraffin) 1:1000, Requires protein digestion pre-treatment of paraffin sections. e.g. trypsin or pronase.
Flow Cytometry: Requires membrane permeabilization.
Optimal working dilutions to be determined by researcher.
Recommended Secondary ReagentsI1904-65W: IgG, F(abí)2 (HRP) Pab RbxMo
I1904-66C: IgG, F(abí)2, X-Adsorbed (HRP) Pab RbxMo
F(ab')2 Rabbit anti mouse IgG: PE
Recommended Positive Control TissueNeutrophils, HL-60 cell line
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No3H2072
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.09% sodium azide and 0.5% BSA.
PurityPurified IgG
SpecificityRecognizes human defensin, expressed by mature neutrophils.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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