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D2675-02E Goat Anti-DAZL (Deleted in Azoospermia-like, DAZ-like Autosomal, Deleted in Azoospermia-like 1, DAZ Homolog, SPGY-like-autosomal, DAZL, DAZH, DAZL1, DAZLA, SPGYLA)

Specifications
References
Clone Type
Polyclonal
Host
Goat
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E IHC WB
Crossreactivity
Bo Ca Hu Mo Rt
Accession #
NP_001342.2
Gene ID
1618
Shipping Temp
Blue Ice
Storage Temp
-20°C

The DAZ (Deleted in AZoospermia) family are potential RNA binding proteins that are expressed in prenatal and postnatal germ cells of males and females. DAZL is localized to the nucleus and cytoplasm of fetal germ cells and to the cytoplasm of developing oocytes. In the testis, this protein is localized to the nucleus of spermatogonia but relocates to the cytoplasm during meiosis where it persists in spermatids and spermatozoa.

Applications
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
ELISA: 1:64,000 Western Blot: 0.1-0.3ug/ml, observed in human ovary testes lysates on ~35kD bands Immunohistochemistry (Paraffin): 1-3ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to C-GNGPQKKSVDR, from DAZL, at the internal region of the protein (NP_001342.2).
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human DAZL. Species Crossreactivity: Mouse. Species sequence homology: Bovine, canine and rat.
References
1. Cauffman G, Van de Velde H, Liebaers I, Van Steirteghem A. DAZL expression in human oocytes, preimplantation embryos and embryonic stem cells. Mol Hum Reprod. 2005 Jun;11(6):405-411. Epub 2005 May 6.
USBio References
No references available
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