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You are here:Home » Antibodies » Antibodies-Notch Proteins » Anti -Delta-like 1 (DLL1, Delta1, DL1, H-Delta-1, UNQ146/PRO172)

Anti -Delta-like 1 (DLL1, Delta1, DL1, H-Delta-1, UNQ146/PRO172)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Sheep Affinity Purified E B IH FC
Delta-1 (also known as Delta-like 1, DLL1) is an 80-100kD vertebrate homolog of the Drosophila delta protein. Rat Delta-1 is a type I transmembrane protein that is 697aa in length. It contains a 520aa extracellular domain (ECD) and a 154aa cytoplasmic region. The ECD has one DSL segment (aa158-220) and eight consecutive EGF-like domains (aa225-515). Proteolytic processing of Delta-1 by ADAMs generates soluble intracellular and extracellular domains. Delta-1 binds to Notch and limits monocyte development while promoting T cell and DC development.
Catalog #D2727-70
ApplicationsSuitable for use in Western Blot, Immunohistochemistry, Direct ELISA and Flow Cytometry. Other applications not tested.
Recommended DilutionsWestern Blot: 0.1ug/ml
Immunohistochemistry: 5-15ug/ml using immersion fixed frozen sections of embryonic mouse stomach
Flow Cytometry: 1ug labels 1x10e6 mouse splenocytes.
Optimal dilutions to be determined by researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostSheep
SourceRat
Concentration~0.2mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
PurityPurified by immunoaffinity chromatography
ImmunogenRecombinant protein corresponding to aa18-537 of rat Delta-like 1 expressed in NS0 cells (P97677).
Species Sequence HomologyMouse; 97%, human; 91%
SpecificityRecognizes the extracellular domain of rat Delta-like 1.
Species CrossreactivityMouse. In Direct ELISA, rhDDL-1
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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