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You are here:Home » Antibodies » Antibodies-Notch Proteins » Anti -Delta-like 1 (DLL1, Delta1, DL1, H-Delta-1, UNQ146/PRO172)

Anti -Delta-like 1 (DLL1, Delta1, DL1, H-Delta-1, UNQ146/PRO172)


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Clone Host Grade Applications
Polyclonal Sheep Affinity Purified E B IH FC
Delta-1 (also known as Delta-like 1, DLL1) is an 80-100kD vertebrate homolog of the Drosophila delta protein. Rat Delta-1 is a type I transmembrane protein that is 697aa in length. It contains a 520aa extracellular domain (ECD) and a 154aa cytoplasmic region. The ECD has one DSL segment (aa158-220) and eight consecutive EGF-like domains (aa225-515). Proteolytic processing of Delta-1 by ADAMs generates soluble intracellular and extracellular domains. Delta-1 binds to Notch and limits monocyte development while promoting T cell and DC development.
Catalog #D2727-70
ApplicationsSuitable for use in Western Blot, Immunohistochemistry, Direct ELISA and Flow Cytometry. Other applications not tested.
Recommended DilutionsWestern Blot: 0.1ug/ml
Immunohistochemistry: 5-15ug/ml using immersion fixed frozen sections of embryonic mouse stomach
Flow Cytometry: 1ug labels 1x10e6 mouse splenocytes.
Optimal dilutions to be determined by researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
Concentration~0.2mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
PurityPurified by immunoaffinity chromatography
ImmunogenRecombinant protein corresponding to aa18-537 of rat Delta-like 1 expressed in NS0 cells (P97677).
Species Sequence HomologyMouse; 97%, human; 91%
SpecificityRecognizes the extracellular domain of rat Delta-like 1.
Species CrossreactivityMouse. In Direct ELISA, rhDDL-1
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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