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You are here:Home » Antibodies » Abs to Notch Proteins » Anti -Delta-like 1 (DLL1, Delta1, DL1, H-Delta-1, UNQ146/PRO172)

Anti -Delta-like 1 (DLL1, Delta1, DL1, H-Delta-1, UNQ146/PRO172)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Sheep Affinity Purified E B IH FC
Delta-1 (also known as Delta-like 1, DLL1) is an 80-100kD vertebrate homolog of the Drosophila delta protein. Rat Delta-1 is a type I transmembrane protein that is 697aa in length. It contains a 520aa extracellular domain (ECD) and a 154aa cytoplasmic region. The ECD has one DSL segment (aa158-220) and eight consecutive EGF-like domains (aa225-515). Proteolytic processing of Delta-1 by ADAMs generates soluble intracellular and extracellular domains. Delta-1 binds to Notch and limits monocyte development while promoting T cell and DC development.
Catalog #D2727-70
ApplicationsSuitable for use in Western Blot, Immunohistochemistry, Direct ELISA and Flow Cytometry. Other applications not tested.
Recommended DilutionsWestern Blot: 0.1-0.2ug/ml; The detection limit is 5ng/lane under non-reducing and 25ng/lane under reducing conditions.
Immunohistochemistry: 10ug/ml detects DLL1 in cryosections of E13.5 mouse embryos. Tissue was fixed overnight in 4% PFA+14% picric acid in PBS at 4°C. Sections were blocked with 10% donkey serum and 0.1% Triton in 1% BSA in PBS for 45 min at RT. After blocking, sections were incubated with D2727-20 for 3 hours at RT followed by fluorochrome-coupled anti-sheep lgG at RT for 1 hour. Between each step, sections were washed with 0.1% BSA in PBS.
Direct ELISA: 0.5-1.0ug/ml; The detection limit is 0.3ng/well.
Flow Cytometry: 25ug/ml; 10ul labels 1-2.5x10e5 cells in total reaction volume < 200ul. Validated using mouse splenocytes.
Optimal dilutions to be determined by researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostSheep
SourceRat
Concentration~0.2mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
PurityPurified by affinity chromatography
ImmunogenRecombinant protein corresponding to aa18-537 of rat Delta-like 1 expressed in NSO cells (P97677).
Species Sequence HomologyMouse; 97%, human; 91%
SpecificityRecognizes the extracellular domain of rat Delta-like 1. Species Crossreactivity
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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