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You are here:Home » Antibodies » Abs to Notch Proteins » Anti -DLL1 (Delta-like Protein 1, Drosophila Delta Homolog 1, Delta1, H-Delta-1, UNQ146/PRO172)

Anti -DLL1 (Delta-like Protein 1, Drosophila Delta Homolog 1, Delta1, H-Delta-1,
UNQ146/PRO172)

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Specifications

Clone Host Grade Applications
Monoclonal Hamster Affinity Purified IH IF
The Notch signalling pathway is an evolutionarily conserved pathway in multi-cellular organisms, which is vital for cell-cell communication, important during fundamental developmental and physiological processes, including regulation of cell fate decisions during neuronal, cardiac and endocrine development, stem cell haematopoiesis, thymic T-cell development, and both tumor progression and suppression. Ligation of Notch receptors by their specific ligands, Jagged1 (CD339), Jagged2, Delta like-1 (DLL1), DLL3 and DLL4, on physically adjacent signal receiving cells, induces proteolysis of the receptors by ADAM-family metalloproteases and gamma-secretase complex, within the transmembrane domain, releasing the Notch intracellular domain (NICD) to translocate to the nucleus. Subsequent signal transduction then occurs through either the CSL-NICD-Mastermind complex cascade (canonical pathway), or NF-kappaB-NICD and CSL-NICD-Deltex complex signalling cascades (non-canonical pathway). The canonical pathway inhibits the differentiation of stem cells or progenitor cells, whilst the non-canonical pathway promotes differentiation. DLL1 is widely expressed, and acts as a mediator of cell fate decisions during haematopoiesis, and may play a role in cell-to-cell communication in mammalian embryos. DLL1 plays an important role in B and T cell differentiation, in embryonic somite formation and patterning, and associates with the scaffolding protein MAGI1 at adherens junctions on neuronal processes. Signalling through DLL1 and Notch 2 has been implicated in the development of marginal zone B cells (MZB).
Catalog #D2727-75D
ApplicationsSuitable for use in Flow Cytometry, Immunohistochemistry. Other applications not tested.
Recommended DilutionFlow Cytometry: 10ul labels 1x10e6 cells in 100ul
Immunohistochemistry: Frozen, Paraffin
Optimal dilutions to be determined by the researcher.
HybridomaP3U1 myeloma cells with spleen cells from Armenian hamsters.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG
Clone No12G26
HostHamster
SourceMouse
Concentration~1mg/ml
FormSupplied as a liquid in PBS, 1mg/ml BSA, 0.9% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenDLL1-expressing CHO cells.
SpecificityRecognizes Delta-like protein 1 (DLL1). Species Crossreactivity: Rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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