Anti -DLL1 (Delta-like Protein 1, Drosophila Delta Homolog 1, Delta1, H-Delta-1,
UNQ146/PRO172)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Hamster |
Affinity Purified |
IH IF |
|
| The Notch signalling pathway is an evolutionarily conserved pathway in multi-cellular organisms, which is vital for cell-cell communication, important during fundamental developmental and physiological processes, including regulation of cell fate decisions during neuronal, cardiac and endocrine development, stem cell haematopoiesis, thymic T-cell development, and both tumor progression and suppression. Ligation of Notch receptors by their specific ligands, Jagged1 (CD339), Jagged2, Delta like-1 (DLL1), DLL3 and DLL4, on physically adjacent signal receiving cells, induces proteolysis of the receptors by ADAM-family metalloproteases and gamma-secretase complex, within the transmembrane domain, releasing the Notch intracellular domain (NICD) to translocate to the nucleus. Subsequent signal transduction then occurs through either the CSL-NICD-Mastermind complex cascade (canonical pathway), or NF-kappaB-NICD and CSL-NICD-Deltex complex signalling cascades (non-canonical pathway). The canonical pathway inhibits the differentiation of stem cells or progenitor cells, whilst the non-canonical pathway promotes differentiation. DLL1 is widely expressed, and acts as a mediator of cell fate decisions during haematopoiesis, and may play a role in cell-to-cell communication in mammalian embryos. DLL1 plays an important role in B and T cell differentiation, in embryonic somite formation and patterning, and associates with the scaffolding protein MAGI1 at adherens junctions on neuronal processes. Signalling through DLL1 and Notch 2 has been implicated in the development of marginal zone B cells (MZB). | | | Catalog # | D2727-75D | | Applications | Suitable for use in Flow Cytometry, Immunohistochemistry. Other applications not tested. | | Recommended Dilution | Flow Cytometry: 10ul labels 1x10e6 cells in 100ul | | Immunohistochemistry: Frozen, Paraffin | | Optimal dilutions to be determined by the researcher. | | Hybridoma | P3U1 myeloma cells with spleen cells from Armenian hamsters. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG | | Clone No | 12G26 | | Host | Hamster | | Source | Mouse | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in PBS, 1mg/ml BSA, 0.9% sodium azide. | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | DLL1-expressing CHO cells. | | Specificity | Recognizes Delta-like protein 1 (DLL1). Species Crossreactivity: Rat. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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