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You are here:Home » Antibodies » Abs to Notch Proteins » Anti -DLL3 (Delta-like Protein 3, Drosophila Delta Homolog 3, Delta3)

Anti -DLL3 (Delta-like Protein 3, Drosophila Delta Homolog 3, Delta3)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Delta-like 3 (DLL3) is a transmembrane Delta-like protein that contains one DSL domain and six EGF-like repeats. DLL3 is critical for somite segmentation and neurogenesis during early embryonic development. It does not bind Notch receptors but functions as an antagonist of Notch signaling. Defects in DLL3 cause axial skeletal abnormalities in spondylocostal dysostosis. Within the extracellular domain, human DLL3 shares 84% amino acid sequence identity with mouse and rat DLL3.
Catalog #D2727-85
ApplicationsSuitable for use in Western Blot and ELISA. Other applications not tested.
Recommended DilutionWestern Blot: 1-2ug/ml. Using a colorimetric detection system, the detection limit for rhDLL3 is ~25ng/lane under reducing and non-reducing conditions. Chemiluminescent detection will increase sensitivity by 5-50 fold.
ELISA: 0.5-1ug/ml. The detection limit for rhDLL3 is ~6ng/well.
Optimal dilutions to be determined by the researcher.
HybridomaA fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, CHO-derived, recombinant human DLL3.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2b
Clone No7H171
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenCHO-derived recombinant human DLL3 (aa27-488) Accession #Q9NYJ7
SpecificityRecognizes human DLL3. Does not recognize rhDLL1 or rhDLL4.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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