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You are here:Home » Antibodies » Abs to Notch Proteins » Anti -Delta 4 (Delta-like protein 4, DLL4, Drosophila Delta homolog 4, notch ligand DLL4 precursor)

Anti -Delta 4 (Delta-like protein 4, DLL4, Drosophila Delta homolog 4, notch ligand DLL4
precursor)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH
Delta 4 (Delta 4 Precursor, Delta Ligand 4 Precursor, Delta-like Protein 4, DLL4, Drosophila Delta Homolog 4, Notch Ligand DLL4 Precursor) is a homolog of the Drosophila delta protein. The delta gene family encodes Notch ligands that are characterized by a DSL domain, EGF repeats, and a
transmembrane domain. DELTA-4 plays a role in the Notch signaling pathway and activates Notch-1 and Notch-4. DELTA-4 is a membrane protein that is expressed in the vascular endothelium.
Catalog #D2728
ApplicationsSuitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:5000-1:25,000
Immunohistochemistry (FFPE): 10-20ug/ml; Detects Delta 4 in a variety of tissues including colon, liver, skeletal muscle, ovary, pancreas, prostate, testes, thymus, tonsil and uterus. In contrast to reports, no staining was observed in vascular tissue.
Western Blot: 1:500-1:2500. Recognizes a band ~74kD.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.01% sodium azide. No stabilizing proteins added.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to an internal region of human Delta 4.
SpecificityRecognizes human Delta 4. Species Sequence Homology: mouse, rat and chimpanzee (91%).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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