Anti -DLL4 (Delta-like Protein 4, Drosophila Delta Homolog 4, Delta4, UNQ1895/PRO4341)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Hamster |
Affinity Purified |
IH FC |
|
| The Notch signaling pathway is an evolutionarily conserved pathway in multicellular organisms, which is vital for cellcell communication, important during fundamental developmental and physiological processes, including regulation of cell fate decisions during neuronal, cardiac and endocrine development, stem cell hematopoiesis, thymic Tcell development, and both tumor progression and suppression. Ligation of Notch receptors by their specific ligands, Jagged1 (CD339), Jagged2, Deltalike protein 1 (DLL1), DLL3 and DLL4, on physically adjacent signal receiving cells, induces proteolysis of the receptors by ADAMfamily metalloproteases and the gammasecretase complex, within the transmembrane domain, releasing the Notch intracellular domain (NICD) to translocate to the nucleus. Subsequent signal transduction then occurs through either the CSLNICDMastermind complex cascade (canonical pathway), or NFkappaBNICD and CSLNICD Deltex complex signaling cascades (noncanonical pathway). The canonical pathway inhibits the differentiation of stem cells or progenitor cells, whilst the noncanonical pathway promotes differentiation. DLL4 is expressed by vascular endothelium, and plays a vital role in embryonic vascular development. DLL4 signaling has been shown to play a role in the angiogenesis of clearcell renal tumors, and pancreatic, bladder and colonic cancer. Studies have shown that DLL4 expression in endothelium cells, can be upregulated by vascular endothelial growth factor (VEGF) and basicFGF, and by HIF1 alpha, and that blockade of DLL4 inhibits tumor growth by promoting nonproductive angiogenesis. | | | Clone HMD42 has been shown to block mouse Notch1Fc and Notch4Fc binding to mouse DLL4:CHO cells, and to inhibit subcutaneous tumor growth in mouse tumor models using the murine lung carcinoma cell line KLN205 (3). Removal of sodium azide is recommended prior to use in functional assays. AbD Serotec recommend the use of EQU003 for this purpose. | | | Catalog # | D2728-01J | | Applications | Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested. | | Recommended Dilution | Flow Cytometry: 10ul labels 1x10e6 cells in 100ul | | Immunohistochemistry: Frozen, Paraffin | | Optimal dilutions to be determined by the researcher. | | Positive Control | Mouse spleen | | Hybridoma | P3U1 myeloma cells with spleen cells from Armenian hamsters. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG | | Clone No | 12G27 | | Host | Hamster | | Source | Mouse | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in PBS, pH 6.0, 0.09% sodium azide. | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | Recombinant corresponding to mouse DLL4. | | Specificity | Recognizes mouse Delta-like protein 4 (DLL4). | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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