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You are here:Home » Antibodies » Abs to Notch Proteins » Anti -Dll-4, ID (Delta-like 4 (Drosophila), dll4)

Anti -Dll-4, ID (Delta-like 4 (Drosophila), dll4)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E
Delta-like protein 4 (DLL4) is a type I membrane protein belonging to the Delta/Serrate/Lag2 (DSL) family of Notch ligands. Notch signaling is an evolutionarily conserved pathway that controls cell fate and is required in multiple developmental processes including vascular development, hematopoiesis, somatogenesis, myogenesis, and neurogenesis. Dysregulation in the Notch pathway is associated with various human diseases. In mammals, four Notch homologs (Notch 1 to 4) and five ligands (DLL 1, 3 and 4, Jagged 1 and 2) have been identified. Notch ligands are transmembrane proteins with a DSL motif necessary for Notch binding, tandem EGF repeats, a transmembrane region and a short intracellular domain (ICD). Notch ligands are categorized into two subfamilies based on the presence of an extracellular cysteine­rich domain and insertions that interrupt some EGF repeats in the Jagged but not the Delta ligand family. Interactions of Notch receptors with their ligands results in reciprocal regulated intramembrane proteolysis (RIP). RIP is a mechanism for transmembrane signal transduction that involves the sequential processing by a disintegrin metalloprotease (ADAM) and then by presenilin/gamma secretase, resulting in shedding of the extracellular domains and the generation of the soluble ICD signaling fragments, respectively. The Notch ICD translocates to the nucleus and interacts with transcriptional coactivators, resulting in the transcription of target genes. Human and mouse DLL4 share 86% aa sequence identity.
Catalog #D2731-08
ApplicationsSuitable for use in ELISA. Other applications not tested.
Recommended DilutionsELISA: 1:5000-1:20,000
Optimal dilutions to be determined by the researcher.
Control Peptide D2731-08P
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceZebrafish
ConcentrationNot Determined
FormSupplied as a liquid in 40mM MOPS, pH 7.5, 0.1% BSA, 0.05% sodium azide, 50% glycerol
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to the intermediate region of zebrafish Dll-4 (NP_001073304.1)
SpecificityRecognizes zebrafish Dll-4.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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