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You are here:Home » Biochemicals » Biochem-Nucleotides » 11-dATP (Biotin) (Biotin-11-dATP)

11-dATP (Biotin) (Biotin-11-dATP)

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Specifications

Biotin-11-dATP is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dATP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal substrate properties and thus labeling efficiency as well as an efficient detection of the Biotin moiety is ensured by a 11-atom linker attached to the 7-Deaza position of adenine.
Catalog #D3165-02
Elemental AnalysisC: ~40%, H: ~5%, N: ~12%, O: ~27%, P: ~10%, S: ~3%
Spectroscopic Properties max 280nm; 12,700
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular FormulaC30H45N8O15P3S
Molecular Weight882.71
Purity~95%
Concentration~1mM
FormSupplied as a liquid in 10mM Tris, pH 7.5. Labeled with Biotin.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesg-[N-(Biotin-6-amino-hexanoyl)]-7-propargylamino-2?-deoxy-7-deaza-adenosine-5?-triphosphate, Triethylammonium Salt


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