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Specifications

Isolation of enteric bacilli and differentiation of lactose fermenters.
Catalog #	D3170
Description	Deoxycholate Agar was first described by Leifson for the isolation of intestinal pathogens and for the enumeration of colon bacilli in milk and water. In the microbiological analysis of dairy products and water, Deoxycholate Agar is normally used for enumeration of conforms in pour plates. It may also be used for isolation of enteric pathogens by surface inoculation from rectal swabs, feces or other specimens. Deoxycholate Agar should be used in parallel with other plating media when used for this purpose. Sodium deoxycholate and sodium citrate are incorporated in the medium to inhibit the growth of Gram positive organisms. Fermentation of lactose causes an acid reaction in the medium resulting in the formation of deep red colonies.
Storage	Store the sealed bottle containing the dehydrated medium at 2 to 30.0°C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the color has changed from the original light pinkish-beige color.
Microbiological Analysis
Escherichia coli ATCC 25922	Growth, Pink to red colonies
Salmonella typhimurium ATCC 14028	Growth Colorless colonies
Staphylococcus aureus ATCC 25923	Inhibited
Appearance
Pinkish-beige, homogeneous, free flowing powder.
Solubility
Brownish-red, clear to slightly turbid.
pH (25°C)
7.3±0.2
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.