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You are here:Home » Culture Media » Media-Microbiological » Deoxycholate Lactose Agar (Powder)

Deoxycholate Lactose Agar (Powder)

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Specifications

Recovery of coliforms in milk and water.
Catalog #D3174
DescriptionDeoxycholate Lactose Agar was formulated by Leifson for the detection of coliforms in water, dairy products and other food materials. USBio recommends Violet Red Bile Agar as the medium of choice for isolating coliforms from food products. Growth of coliforms which ferment lactose produce red colonies surrounded by a zone of red precipitate. The fermentation of lactose produces acid products which lowers the pH of the medium and the neutral red dye in the medium turns bright red. Sodium citrate and sodium deoxycholate are added to inhibit the growth of Gram positive bacteria.
StorageStore the sealed bottle containing the dehydrated medium at 2 to 30.0°C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the color has changed from the original light pinkish-beige color.
Microbiological Analysis
Escherichia coli ATCC 25922Growth Pink w/ bile ppt.
Salmonella typhimurium ATCC 14028Growth Colorless
Enterococcus faecalis ATCC 29212Inhibited.
Appearance
Light pinkish-beige, homogeneous, free flowing powder.
Solubility
Reddish-pink, clear, complete.
pH (25°C)
7.1±0.2
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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