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You are here:Home » Cloning » Cloning-Nucleotides » 2’-Deoxynucleotide Triphosphates (Set of 4 dNTP’s) dATP, dCTP, dGTP, dTTP Solution, BioGenomics™

2’-Deoxynucleotide Triphosphates (Set of 4 dNTP’s) dATP, dCTP, dGTP, dTTP Solution,
BioGenomics™

Pricing

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Specifications

Suitable for use in PCR™, cDNA synthesis, DNA sequencing and labeling procedures.
Supplied as a set of 4 dNTP’s at a concentration of 100mM.
Functionally tested in PCR with Taq and Pfu DNA Polymerases.
Catalog #D3191
ComponentsD3205: 2’-Deoxythymidine-5’-Triphosphate, Sodium Salt (dTTP) Solution:
Purity (HPLC): 99%
Concentration: 100mM ± 5%
pH: 7.0 ± 0.1
Lambda Max: 260nm
Extinction Coefficient: 9600 (pH 7, 20mM potassium phosphate)
A250/A260: 0.65 ± 0.03 A280/A260: 0.73 ± 0.03
DNase: None Detected RNase: None Detected
D3165 2’-Deoxyadenosine-5’-Triphosphate, Sodium Salt Solution (dATP):
Purity (HPLC): 99%
Concentration: 100mM ± 5%
pH: 7.0 ± 0.1
Lambda Max: 259nm ± 2nm
Extinction Coefficient: 15200 (pH 7, 20mM potassium phosphate)
A250/A260: 0.80 ± 0.03 A280/A260: 0.15 ± 0.03
DNase: None Detected RNase: None Detected
D3187 2’-Deoxycytidine-5’-Triphosphate, Sodium Salt (dCTP) Solution:
Purity (HPLC): 99%
Concentration: 100mM ± 5%
pH: 7.0 ± 0.1
Lambda Max: 272nm ± 2nm
Extinction Coefficient: 9300 (pH 7, 20mM potassium phosphate)
A250/A260: 0.80 ± 0.03 A280/A260: 0.96 ± 0.03
DNase: None Detected RNase: None Detected
D3189 2’-Deoxyguanosine-5’-Triphosphate, Sodium Salt (dGTP) Solution:
Purity (HPLC): 99%
Concentration: 100mM ± 5%
pH: 7.0 ± 0.1
Lambda Max: 253nm
Extinction Coefficient: 13700 (pH 7, 20mM potassium phosphate)
A250/A260: 1.15 ± 0.03 A280/A260: 0.67 ± 0.03
DNase: None Detected RNase: None Detected
Storage and StabilityMay be stored at -20°C for short-term only. For long-term storage, aliquot and store at -70°C. Aliquots are stable for at least 6 months at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
™The Polymerase Chain Reaction (PCR) process is covered by patents owned by Hoffman-La Roche.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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