| Suitable for use in PCR™, cDNA synthesis, DNA sequencing and labeling procedures. |
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| Supplied as a set of 4 dNTP’s at a concentration of 100mM. |
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| Functionally tested in PCR with Taq and Pfu DNA Polymerases. |
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| Catalog # | D3191 |
| Components | D3205: 2’-Deoxythymidine-5’-Triphosphate, Sodium Salt (dTTP) Solution: |
| Purity (HPLC): 99% |
| Concentration: 100mM ± 5% |
| pH: 7.0 ± 0.1 |
| Lambda Max: 260nm |
| Extinction Coefficient: 9600 (pH 7, 20mM potassium phosphate) |
| A250/A260: 0.65 ± 0.03 A280/A260: 0.73 ± 0.03 |
| DNase: None Detected RNase: None Detected |
| D3165 | 2’-Deoxyadenosine-5’-Triphosphate, Sodium Salt Solution (dATP): |
| Purity (HPLC): 99% |
| Concentration: 100mM ± 5% |
| pH: 7.0 ± 0.1 |
| Lambda Max: 259nm ± 2nm |
| Extinction Coefficient: 15200 (pH 7, 20mM potassium phosphate) |
| A250/A260: 0.80 ± 0.03 A280/A260: 0.15 ± 0.03 |
| DNase: None Detected RNase: None Detected |
| D3187 | 2’-Deoxycytidine-5’-Triphosphate, Sodium Salt (dCTP) Solution: |
| Purity (HPLC): 99% |
| Concentration: 100mM ± 5% |
| pH: 7.0 ± 0.1 |
| Lambda Max: 272nm ± 2nm |
| Extinction Coefficient: 9300 (pH 7, 20mM potassium phosphate) |
| A250/A260: 0.80 ± 0.03 A280/A260: 0.96 ± 0.03 |
| DNase: None Detected RNase: None Detected |
| D3189 | 2’-Deoxyguanosine-5’-Triphosphate, Sodium Salt (dGTP) Solution: |
| Purity (HPLC): 99% |
| Concentration: 100mM ± 5% |
| pH: 7.0 ± 0.1 |
| Lambda Max: 253nm |
| Extinction Coefficient: 13700 (pH 7, 20mM potassium phosphate) |
| A250/A260: 1.15 ± 0.03 A280/A260: 0.67 ± 0.03 |
| DNase: None Detected RNase: None Detected |
| Storage and Stability | May be stored at -20°C for short-term only. For long-term storage, aliquot and store at -70°C. Aliquots are stable for at least 6 months at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| ™The Polymerase Chain Reaction (PCR) process is covered by patents owned by Hoffman-La Roche. |
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
