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You are here:Home » Molecular Biology » Cloning-Enzymes » Deoxyribonuclease I (DNase I), 1500u/mg, Lyophilized

Deoxyribonuclease I (DNase I), 1500u/mg, Lyophilized

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Specifications

Endonuclease which digests dsDNA by hydrolysis of deoxyribonucleotide linkages, producing 3-hydroxyl oligonucleotides. Supplied as a chromatographically purified, lyophilized powder.
Catalog #D3200-01
ApplicationsNick Translation
DNase I DNA Footprinting
Cloning of Random DNA Fragments
SourceBovine pancreas
FormSupplied as a chromatographically purified, salt-free, off-white lyophilized powder.
Activity 1500u/mg
Unit Definition1 unit causes an increase in absorbance at 260nm of 0.001 per minute per ml at 25ºC when acting upon highly polymerized DNA at pH 5.0.
Note: 0.005 Kunitz units digests 1ug DNA in 10 minutes at 37°C, 50mM Tris, 1mM Ca+2, 1mM Mg+2 in a 50ul reaction.
Storage and StabilityLyophilized powder may be stored at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot and store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Certificate of OriginThe raw animal product used in the manufacturing of Deoxyribonuclease I (DNase I) is derived from animal material originating in New Zealand, Uruguian or Brazil where there have been no reported cases of bovine spongiform encephalopathy (BSE). During processing, the product is subjected to a pH of 1.8 for a minimum period of 14 hours.
CAS Number9003-98-9
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesEC=3.1.21.1; 5?-Oligonucleotidohydrolase


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