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You are here:Home » Molecular Biology » Cloning-Enzymes » Deoxyribonuclease I (DNase I), 2u/ul, Solution

Deoxyribonuclease I (DNase I), 2u/ul, Solution

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Specifications

Endonuclease which digests dsDNA by hydrolysis of deoxyribonucleotide linkages, producing 3-hydroxyl oligonucleotides. Supplied as a chromatographically purified, ribonucleasease & protease-free solution in glycerol.
Catalog #D3200-02
ApplicationsNick Translation
DNase I DNA Footprinting
Cloning of Random DNA Fragments
CAS Number9003-98-9
Source
Bovine pancreas
Form
Supplied as a chromatographically purified, solution in 50% glycerol, 1mM calcium chloride.
Activity
~2u/ul
Unit Definition
1 unit causes an increase in absorbance at 260 nm of 0.001 per minute per ml at 25ºC when acting upon highly polymerized DNA at pH 5.0. Note0.005 Kunitz units digests 1µg DNA in 10 minutes at 37°C, 50mM Tris, 1mM Ca+2, 1mM Mg+2 in a 50µl reaction.
Assay Method
Based on the Kunitz method where DNase will depolymerize DNA; 0.005 Kunitz units digests 1µg of lambda DNA in 10 minutes at 37°C in 50mM Tris, 1mM Ca+2 and 1mM Mg+2, pH 7.8 in a 50µl reaction.
Quality Control
SDS-PAGE
RNaseNone Detected
ProteaseNone Detected
Storage and Stability
May be stored at 4°C for short-term only. Protect from moisture. For long-term storage, store at -20°C. Aliquots with 50% glycerol are stable for at least 6 months at -20°C.
NOTEPancreatic DNase is highly susceptible to denaturation. Mix by gentle inversion.
Alternate namesEC=3.1.21.1; 5?-Oligonucleotidohydrolase


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