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Deoxyribonuclease I (DNase I), 2u/ul, Solution
Deoxyribonuclease I (DNase I), 2u/ul, Solution
Pricing
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| Endonuclease which digests dsDNA by hydrolysis of deoxyribonucleotide linkages, producing 3’-hydroxyl oligonucleotides. Supplied as a chromatographically purified, ribonucleasease & protease-free solution in glycerol. | | | Catalog # | D3200-02 | | Applications | Nick Translation | | DNase I DNA Footprinting | | Cloning of Random DNA Fragments | | CAS Number | 9003-98-9 | | Source | | | Bovine pancreas | | | Form | | | Supplied as a chromatographically purified, solution in 50% glycerol, 1mM calcium chloride. | | | Activity | | | ~2u/ul | | | Unit Definition | | | 1 unit causes an increase in absorbance at 260 nm of 0.001 per minute per ml at 25ºC when acting upon highly polymerized DNA at pH 5.0. Note | 0.005 Kunitz units digests 1µg DNA in 10 minutes at 37°C, 50mM Tris, 1mM Ca+2, 1mM Mg+2 in a 50µl reaction. | | | Assay Method | | | Based on the Kunitz method where DNase will depolymerize DNA; 0.005 Kunitz units digests 1µg of lambda DNA in 10 minutes at 37°C in 50mM Tris, 1mM Ca+2 and 1mM Mg+2, pH 7.8 in a 50µl reaction. | | | Quality Control | | | SDS-PAGE | | RNase | None Detected | | Protease | None Detected | | | Storage and Stability | | | May be stored at 4°C for short-term only. Protect from moisture. For long-term storage, store at -20°C. Aliquots with 50% glycerol are stable for at least 6 months at -20°C. | | | NOTE | Pancreatic DNase is highly susceptible to denaturation. Mix by gentle inversion. | | Alternate names | EC=3.1.21.1; 5?-Oligonucleotidohydrolase |
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