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You are here:Home » Antibodies » Abs to Enzymes, Deoxyribonuclease » Anti -Deoxyribonuclease I (DNase I)

Anti -Deoxyribonuclease I (DNase I)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
Deoxyribonuclease I (DNase I) is a Ca 2+ and Mg 2+ dependent endonuclease. DNase I is synthesized in the pancreas and stored in zymogen granules. It has been used to reduce the viscosity of cystic fibrosis sputum. A DNase I-like enzyme appears to catalyze the degradation of chromatin to oligo- and mononucleosomes during apoptosis. A recent study has demonstrated an endonuclease with activity and antigenicity indistinguishable from DNase I in thymocytes, cells susceptible to apoptosis.
Catalog #D3200-05A
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:10,000-1:50,000
Western Blot: 1:500-1:2000; D3200-05B can be used as a positive control.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceBovine
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenPurified bovine pancreatic DNase I (EC 3.1.21.1).
SpecificityRecognizes bovine Deoxyribonuclease I. Expected to crossreact with other mammalian DNases from human, mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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