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You are here:Home » Antibodies » Abs to Enzymes, Deoxyribonuclease » Anti -Deoxyribonuclease I (DNase I)

Anti -Deoxyribonuclease I (DNase I)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Highly Purified E B IP
Deoxyribonuclease I (DNase I) is a Ca 2+ and Mg 2+ dependent endonuclease. DNase I is synthesized in the pancreas and stored in zymogen granules. It has been used to reduce the viscosity of cystic fibrosis sputum. A DNase I-like enzyme appears to catalyze the degradation of chromatin to oligo-and mononucleosomes during apoptosis. A recent study has demonstrated an endonuclease with activity and antigenicity indistinguishable from DNase I in thymocytes, cells susceptible to apoptosis.
Catalog #D3200-06
ApplicationsSuitable for use in ELISA, Western Blot, Immunoprecipitation and Conjugation. Other applications have not been tested.
Recommended DilutionELISA: 1:500 to 1:1500
Optimal dilutions to be detemined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceBovine
Concentration~10mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.01% sodium azide, before the addition of 40% glycerol.
PurityPurified by Ion Exchange chromatography.
ImmunogenBovine pancreatic Deoxyribonuclease I
SpecificityRecognizes 31kD bovine Deoxyribonuclease I. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum and well as purified and partially purified deoxyribonuclease I, (bovine pancreas).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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