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You are here:Home » Antibodies » Abs to Enzymes, Deoxyribonuclease » Anti -Deoxyribonuclease I (DNase I) (HRP)

Anti -Deoxyribonuclease I (DNase I) (HRP)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Highly Purified E B IP IH
Deoxyribonuclease I (DNase I) is a Ca 2+ and Mg 2+ dependent endonuclease. DNase I is synthesized in the pancreas and stored in zymogen granules. It has been used to reduce the viscosity of cystic fibrosis sputum. A DNase I-like enzyme appears to catalyze the degradation of chromatin to oligo-and mononucleosomes during apoptosis. A recent study has demonstrated an endonuclease with activity and antigenicity indistinguishable from DNase I in thymocytes, cells susceptible to apoptosis.
Catalog #D3200-08
ApplicationsSuitable for use in ELISA, Western Blotting, Immunohistochemistry, and Peroxidase-based assays. Other applications have not been tested.
Recommended DilutionELISA: 1:500 to 1:2500
Optimal dilutions to be determined by the researcher.
LabelHorseradish Peroxidase (HRP).
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceBovine
Concentration~10mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.01% gentamicin sulfate, 10mg/ml BSA, 40% glycerol. Conjugated with horseradish peroxidase.
PurityChromatographically purified, delipidated and defibrinated IgG.
ImmunogenBovine pancreatic Deoxyribonuclease I
SpecificityRecognizes 31kD bovine Deoxyribonuclease I. Crossreactive against mammalian forms. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti peroxidase, anti rabbit serum and purified and partially purified deoxyribonuclease I (bovine pancreas).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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