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You are here:Home » Cloning » Cloning-Enzymes » Deoxyribonuclease II, Porcine spleen (DNase II)

Deoxyribonuclease II, Porcine spleen (DNase II)

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Specifications

Deoxyribonuclease II is a glycoprotein endonuclease with a dimeric structure. Deoxyribonuclease II from porcine spleen has a molecular weight of 38kD. Optimum pH range is 4.5-5.0 at ionic strength 0.15M. Deoxyribonuclease II (Acid DNase) hydrolyzes deoxyribonucleotide linkages in native and denatured DNA yielding products with 3'-phosphates. It also acts on p-nitrophenylphosphodiesters at pH 5.6-5.9. Bernardi (BBRC, 17, 573, 1971) describes a three stage degradation of native DNA by DNase II.
Catalog #D3201
ApplicationsDNA Sequencing (acidic pH)
DNA Digestion (acidic pH)
SourcePorcine pancreas
FormSupplied as a chromatographically purified, dialyzed, lyophilized powder.
Activity 12,000 units per vial
Unit Definition1 unit causes an increase in absorbance at 260 nm of 0.002 per minute per ml at 25ºC when acting upon highly polymerized DNA at pH 4.6.
Note: 0.005 Kunitz units digests 1ug DNA in 10 minutes at 37°C, 50mM Tris, 1mM Ca+2, 1mM Mg+2 in a 50ul reaction.
Assay MethodBased on the Kunitz method where DNase will depolymerize DNA; 0.005 Kunitz units digests 1ug of lambda DNA in 10 minutes at 37°C in 50mM Tris, 1mM Ca+2 and 1mM Mg+2, pH 7.8 in a 50ul reaction.
Quality ControlSDS-PAGE
Storage and StabilityStable for 6-12 months at -20ºC
Country of OriginUS certified starting material, collected in USDA approved facilities
CAS Number9003-98-9
Molecular Weight38kD
SourcePorcine spleen
PurityChromatographically purified in a modification of the procedure of Bernardi, et al., BBA, 129, 1 (1966).
FormSalt-free, dialyzed, lyophilized powder
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesEC=3.1.22.1; 5?-Oligonucleotidohydrolase


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