| Deoxyribonuclease II is a glycoprotein endonuclease with a dimeric structure. Deoxyribonuclease II from porcine spleen has a molecular weight of 38kD. Optimum pH range is 4.5-5.0 at ionic strength 0.15M. Deoxyribonuclease II (Acid DNase) hydrolyzes deoxyribonucleotide linkages in native and denatured DNA yielding products with 3'-phosphates. It also acts on p-nitrophenylphosphodiesters at pH 5.6-5.9. Bernardi (BBRC, 17, 573, 1971) describes a three stage degradation of native DNA by DNase II. |
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| Catalog # | D3201 |
| Applications | DNA Sequencing (acidic pH) |
| DNA Digestion (acidic pH) |
| Source | Porcine pancreas |
| Form | Supplied as a chromatographically purified, dialyzed, lyophilized powder. |
| Activity | 12,000 units per vial |
| Unit Definition | 1 unit causes an increase in absorbance at 260 nm of 0.002 per minute per ml at 25ºC when acting upon highly polymerized DNA at pH 4.6. |
| Note: 0.005 Kunitz units digests 1ug DNA in 10 minutes at 37°C, 50mM Tris, 1mM Ca+2, 1mM Mg+2 in a 50ul reaction. |
| Assay Method | Based on the Kunitz method where DNase will depolymerize DNA; 0.005 Kunitz units digests 1ug of lambda DNA in 10 minutes at 37°C in 50mM Tris, 1mM Ca+2 and 1mM Mg+2, pH 7.8 in a 50ul reaction. |
| Quality Control | SDS-PAGE |
| Storage and Stability | Stable for 6-12 months at -20ºC |
| Country of Origin | US certified starting material, collected in USDA approved facilities |
| CAS Number | 9003-98-9 |
| Molecular Weight | 38kD |
| Source | Porcine spleen |
| Purity | Chromatographically purified in a modification of the procedure of Bernardi, et al., BBA, 129, 1 (1966). |
| Form | Salt-free, dialyzed, lyophilized powder |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
| Alternate names | EC=3.1.22.1; 5?-Oligonucleotidohydrolase |
