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You are here:Home » Antibodies » Abs to Enzymes, Deoxyribonuclease » Anti -Deoxyribonuclease II (Deoxyribonuclease-II, DNase II, DNaseII, Deoxyribonuclease 2, Deoxyribonuclease-2, DNase 2, DNase2, Acid DNase, Deoxyribonuclease 2 alpha, DNase2A, DNL, DNL 2, DNL2, Lysosomal DNase II, R31240 2, R31240_2)

Anti -Deoxyribonuclease II (Deoxyribonuclease-II, DNase II, DNaseII, Deoxyribonuclease 2,
Deoxyribonuclease-2, DNase 2, DNase2, Acid DNase, Deoxyribonuclease 2 alpha, DNase2A, DNL,
DNL 2, DNL2, Lysosomal DNase II, R31240 2, R31240_2)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Purified B
Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins including AIF and Acinus (1). DNase II causes both chromatin condensation and DNA fragmentation (1). The genes encoding human (2-4), porcine (4), and murine (5) DNase II have been cloned. The DNase II gene encodes a 40 kDa proenzyme. The mature enzyme consists of two non-identical subunits, the 32 kDa (alpha) and 12 kDa (beta) chains, generated by proteolytic processing. Overexpression of DNase II induces chromatin condensation (3). DNase II is ubiquitously expressed in human tissues.
Applications:
Suitable for use in Western Blot. Other applications not tested.
Catalog #D3201-02A
Recommended DilutionWestern Blot: 1:500-1:1000 dilution. Human spleen tissue lysate or THP-1 cell lysate can be used as positive control and an approximate 40 kDa band can be detected, which represents the pro-enzyme of DNase II.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
PurityPurified immunoglobulin
ImmunogenPeptide corresponding to amino acids 347 to 360 of human DNase II precursor.
SpecificityRecognizes human DNase II.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesDeoxyribonuclease II alpha, DNase II alpha, Deoxyribonuclease II Lysosomal, DNase II Lysosomal


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