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You are here:Home » Antibodies » Abs to Enzymes, Deoxyribonuclease » Anti -Deoxyribonuclease II (DNase II)

Anti -Deoxyribonuclease II (DNase II)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E
Deoxyribonuclease II (Dnase II, EC.3.1.22.1) is one of the two Dnase present in mammalian tissues and biological fluids. Dnase II hydrolyzes DNA to 3’-phosphoryl oligonucleotides under acidic conditions. Dnase II is also known as “acid Dnase”. It has preference of for dsDNA. Dnase II has been implicated in internucleosomal cleavage of chromatin to generate DNA fragments or ladder, a hallmark of apoptosis. Dnase II (mouse 353aa; human 360aa, chromosome 19p13.2-q13.4) is a lysosomal enzyme present in most tissues. Dnase Ii is synthesized as a pro-enzyme (signal peptide aa1-16, propeptide region of 91-aa, and 253-aa mature protein (aa110-353) and processed to produce active enzyme containing 2- chains of ~32 and 12kD. Dnase II has no significant sequence homology with Dnase I.
Catalog #D3201-32
ApplicationsSuitable for use in ELISA. Other applications not tested.
Recommended DilutionsELISA: 1:10,000-50,000 using 50-100ng control peptide/well.
Optimal dilutions to be determined by the researcher.
Control PeptideD3201-30: Deoxyribonuclease II, Mouse, Control Peptide (DNase II)
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.1% BSA
PurityPurified by immunoaffinity chromatography
ImmunogenA synthetic peptide corresponding to 17aa near the C-terminus of mouse Dnase II, conjugated to KLH
SpecificityRecognizes mouse Deoxyribonuclease II (Dnase II). The peptide sequence is not well conserved in human Dnase II. No significant sequence homology with other lysosomal enzymes.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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