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You are here:Home » Antibodies » Abs to Extracellular Matrix Proteins » Anti -Desmin (DES, CMD1I, CSM1, CSM2, FLJ12025, FLJ39719, FLJ41013, FLJ41793, Intermediate Filament Protein)

Anti -Desmin (DES, CMD1I, CSM1, CSM2, FLJ12025, FLJ39719, FLJ41013, FLJ41793, Intermediate
Filament Protein)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH
Desmin (53kD) exhibits a high degree of tissue specificity, its expression being predominantly confined to all types of muscle cells (cardiac, skeletal and smooth muscle). Regulation of Desmin expression is stage and tissue-specific, since it is induced during terminal development of, for example, skeletal muscle cell differentiation. In skeletal and cardiac muscle cells Desmin is localized in the Z-disk region and at the intercalated disk. The expression pattern of Desmin in smooth muscle is much more heterogenous.
Coexpression of Vimentin and Desmin has been observed in tumors derived from muscle tissue, i.e. rhabdomyosarcomas and leiomyosarcomas. Furthermore, during myocard disfunction dramatic changes in the distribution of Desmin have been observed.
Catalog #D3221-05
ApplicationsSuitable for use in Immunohistochemistry and Western Blot. Other applications not tested.
Recommended DilutionImmunohistochemistry (Frozen): 1:50-1:100 using ABC method.
Western Blot: 1:50-1:500
Optimum dilutions to be determined by researcher.
HybridomaSp2/0-Ag14 myeloma cells with spleen cells from a mouse immunized with a cytoskeletal Desmin extract of chicken gizzard.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2b
Clone No4A44
HostMouse
SourceChicken
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, 0.09% sodium azide, before the addition of glycerol to 40%.
PurityPurified by Protein G affinity chromatography.
ImmunogenCytoskeletal Desmin extract of chicken gizzard
SpecificityRecognizes chicken Desmin, which is expressed in smooth and striated muscle cells and their tumors, i.e. rhabdomyosarcoma and leiomyosarcoma. Species Crossreactivity: Human, mouse, rat, hamster, canine, porcine, rabbit.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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