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You are here:Home » Antibodies » Abs to Extracellular Matrix Proteins » Anti -Desmin (DES, CMD1I, CSM1, CSM2, FLJ12025, FLJ39719, FLJ41013, FLJ41793, Intermediate Filament Protein)

Anti -Desmin (DES, CMD1I, CSM1, CSM2, FLJ12025, FLJ39719, FLJ41013, FLJ41793, Intermediate
Filament Protein)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Supernatant B IH IF
Desmin is the main intermediate filament in mature skeletal, cardiac and smooth-muscle cells. Can be used to distinguish rhabdomyosarcomas and leiomyosarcomas from other soft tissue tumors, lymphomas and carcinomas.
Catalog #D3221-06A
ApplicationsSuitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunohistochemistry (frozen/paraffin): 1:10-1:20 No proteolytic treatment required. (Depending on the secondary system used dilution could be 5-10 times higher, but optimal dilution should always be tested by serial dilution).
Optimal dilution to be determined by researcher.
Positive ControlSkeletal and smooth muscle myosarcomas
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No5F179
HostMouse
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid. Dilute immediately before use with PBS or TBS.
PuritySupernatant
ImmunogenIsolated from human leiomyoma.
SpecificitySpecifically reacts with the cytoplasmic 53kD human desmin protein.
Species CrossreactivityMost mammals, including mouse, rat and rabbit. Does not crossreact with other intermediate filament proteins and is useful for the identification of myodifferentiation (also in tumors).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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