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You are here:Home » Antibodies » Abs to Extracellular Matrix Proteins » Anti -Desmin (DES, CMD1I, CSM1, CSM2, FLJ12025, FLJ39719, FLJ41013, FLJ41793, Intermediate Filament Protein)

Anti -Desmin (DES, CMD1I, CSM1, CSM2, FLJ12025, FLJ39719, FLJ41013, FLJ41793, Intermediate
Filament Protein)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Ascites B IH IC IF
Desmin is the protein subunit of muscle-type intermediate filaments (IFs). Desmin is one of the five major groups of IFs and is found predominantly in skeletal, cardiac, and smooth muscle. This antibody may be used for immunocytochemical localization of intermediate filaments of the Desmin group in all types of muscle cells and to localize Desmin at the periphery of z-discs.
Catalog #D3221-10A
ApplicationsSuitable for use in Immunofluorescence, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended DilutionImmunofluorescence: 1:20
Western Blot: Detects a band at ~50-55kD.
Immunohistochemistry: Paraffin sections.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Numbern/a
Clone TypeMonoclonal
IsotypeIgG1
Clone No8J1272
HostMouse
SourcePorcine
Concentration~8.0mg/ml
FormSupplied as a liquid with 15mM sodium azide.
PurityAscites
ImmunogenFull length native porcine Desmin (purified). Cellular Localization: Cytoplasmic.
SpecificityRecognizes porcine Desmin. Labels Desmin in tumors derived from muscle tissue (leiomyomas and rhabdomyosarcomas). Species Crossreactivity: Feline, chicken, bovine, goat, hamster, human, mouse, rabbit, rat, sheep, snake and lizard.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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