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You are here:Home » Antibodies » Abs to Proteins » Anti -Desmoglein 3

Anti -Desmoglein 3

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IH IF
Desmoglein 3 is a member of the desmosomal cadherin family that comprise the desmogleins and desmocollins. Desmosomes are cell-cell junctions in most epithelial cells and serve to link the intermediate filament cytoskeletons of adjacent cells. Within the desmosome the extracellular domain of desmoglein is essential for calcium dependent heterophilic binding to the desmocollins, whereas the intracellular domain is essential for binding to the desmosomal plaque protein, plakoglobin. Desmoglein 3 is synthesized in both the basal and the lower suprabasal compartments. Domain specific desmosomal and adherens junction-associated antibodies have been widely used in recent studies. In one study, domain specific antibodies were used to determine the cellular localization of the skin of patients with Hailey-Hailey disease and Darierís disease. Desmosomal cadherin antibodies are also useful for targeting specific disease and in the study of interaction between cell adhesion proteins.
Catalog #D3221-84
ApplicationsSuitable for use in Western Blot, Immunoprecipitation, Immunofluorescence and Immunohistochemistry. Other applications not tested.
Recommended DilutionsWestern Blot: 1-3ug/ml using ECL
Immunoprecipitation (Native): 5-10ug
Immunohistochemistry (Paraffin): 20-50ug/ml
Optimal dilutions to be determined by the researcher.
Positive ControlA431 cells
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No3G133
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenHuman desmoglein 3 extracellular domain.
SpecificityRecognizes the human Desmoglein 3 protein at ~140kD. Does not crossreact with desmoglein 1 or desmoglein 2 protein.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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