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You are here:Home » Antibodies » Abs to Proteins » Anti -Desmoplakin 1,2

Anti -Desmoplakin 1,2

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH IF
Desmoplakin (DP) is a cytoplasmic plaque protein that is located in desmosomes, which are abundant in tissues subjected to mechanical stress, like muscle and epidermis. The role of DP is to link the transmembrane cadherins via plakoglobin to the cytoplasmic intermediate filament network (Kowalczyk et al. 1997; Smith and Fuchs 1998). DP comprises an N-terminal, desmosome-associated plakin domain binding to plakoglobin and plakophilin (Smith and Fuchs 1998), a central coiled-coil rod domain responsible for dimerization, and a C-terminal domain consisting of three homologous plakin repeat subdomains, named A, B, and C, that interact with the intermediate filaments (Choi et al. 2002). Alternative splicing generates two isoforms (DP I and DP II) that differ in their rod domain lengths, with relative molecular weights of 332 kD and 259 kD, respectively (Green et al. 1988).
Catalog #D3221-92
Mutations in desmoplakin can cause cardiocutaneous syndromes (for review, see Cheong et al. 2005). Haplotype insufficiency of DP may cause striate palmoplantar keratoderma (PPK [MIM 125647]) (Armstrong et al. 1999; Whittock et al. 1999), whereas dominant missense mutations in the N-terminal domain may cause arrhythmogenic right ventricular dysplasia (ARVD [MIM 607450]) (Rampazzo et al. 2002). A nonsense mutation in the N-terminal domain combined with a recessive missense mutation causes skin fragility/woolly hair syndrome with striate PPK [MIM 607655] (Whittock et al. 2002), whereas a homozygous recessive missense mutation in the B-subdomain causes ARVD, woolly hair, and acral skin blistering (Naxos-like disease) (Alcalai et al. 2003), and a homozygous truncatious mutation in the C-terminus causes left ventricular cardiomyopathy, woolly hair, and PPK (Carvajal syndrome [MIM 605676]) (Norgett et al. 2000). DP must be indispensable, because DP-/- mice are early abortive (Gallicano et al. 1998).
ApplicationsSuitable for use in Western Blot, Immunofluorescence and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionImmunohistochemistry (Frozen): 1:10 with PBS, pH 7.4; Incubation Time 1 hr at RT.
Optimal dilutions to be determined by the researcher.
Reactivities on Cultured Cell LinesSeveral human carcinoma cell lines: MCF-7 and A-431.
Rat MH1C1 cell line; Bovine cells: MDBK and BMGE.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Clone TypeMonoclonal
IsotypeIgG1
Clone No5F190
HostMouse
SourceBovine
Concentration~0.05mg/ml
FormSupplied as a lyophilized powder from PBS, pH 7.4, 0.5% BSA, 0.09% sodium azide. Reconstitute with 1ml ddH2O.
PurityPurified by Protein A affinity chromatography.
ImmunogenBovine desmoplakin 1&2
SpecificityRecognizes bovine desmoplakin 1 and 2 at ~210 and 215kD. Reacts with the primary and metastatic carcinoma and meningioma. Species Crossreactivity: human, rat, mouse and chicken.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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