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You are here:Home » Antibodies » Antibodies-Cell Markers » Anti -Developmental Pluripotency-Associated Protein 5 (DPPA5, ECAT2, Esg-1) (Biotin)

Anti -Developmental Pluripotency-Associated Protein 5 (DPPA5, ECAT2, Esg-1) (Biotin)


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Clone Host Grade Applications
Polyclonal Goat Affinity Purified B
DPPA5, also known as Esg-1 and ECAT2, is a 14kD RNA-binding protein expressed in early embryos, primordial germ cells, and embryonic stem cells and is used as a marker of the pluripotent state. Mouse DPPA5 is 118 amino acids in length and shows 63% amino acid identity with human and 81% amino acid identity with rat DPPA5 proteins.
Catalog #D3221-94T1
ApplicationsWestern Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect mouse DPPA5. The detection limit for rmDPPA5 is approximately 5 ng/lane under non-reducing and reducing conditions. In this format, this antibody shows approximately 25% cross-reactivity with rhDPPA5 and less than 10% cross-reactivity with rmDPPA4.
Optimal dilutions should be determined by each laboratory for each application.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile TBS with 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
ConcentrationAs reported
FormSupplied as a lyophilized powder from a 0.2um filtered solution in PBS. Reconstitute with sterile TBS, pH 7.3 (20mM Trizma base, 150mM sodium chloride) containing 0.1% BSA, 40-50% glycerol. If 1ml of buffer is used, the concentration will be 50ug/ml.
PurityPurified by mouse DPPA5 affinity chromatography and then biotinylated.
ImmunogenE. coli-derived rmDPPA5
SpecificitySelected for use as a detection antibody in mouse DPPA5 Western Blots.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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