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Anti -Developmental Pluripotency-Associated Protein 5 (DPPA5, ECAT2, Esg-1) (Biotin)
Anti -Developmental Pluripotency-Associated Protein 5 (DPPA5, ECAT2, Esg-1) (Biotin)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Goat |
Affinity Purified |
B |
|
| DPPA5, also known as Esg-1 and ECAT2, is a 14kD RNA-binding protein expressed in early embryos, primordial germ cells, and embryonic stem cells and is used as a marker of the pluripotent state. Mouse DPPA5 is 118 amino acids in length and shows 63% amino acid identity with human and 81% amino acid identity with rat DPPA5 proteins. | | | Catalog # | D3221-94T1 | | Applications | Western Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect mouse DPPA5. The detection limit for rmDPPA5 is approximately 5 ng/lane under non-reducing and reducing conditions. In this format, this antibody shows approximately 25% cross-reactivity with rhDPPA5 and less than 10% cross-reactivity with rmDPPA4. | | Optimal dilutions should be determined by each laboratory for each application. | | Storage and Stability | Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile TBS with 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Goat | | Source | Mouse | | Concentration | As reported | | Form | Supplied as a lyophilized powder from a 0.2um filtered solution in PBS. Reconstitute with sterile TBS, pH 7.3 (20mM Trizma base, 150mM sodium chloride) containing 0.1% BSA, 40-50% glycerol. If 1ml of buffer is used, the concentration will be 50ug/ml. | | Purity | Purified by mouse DPPA5 affinity chromatography and then biotinylated. | | Immunogen | E. coli-derived rmDPPA5 | | Specificity | Selected for use as a detection antibody in mouse DPPA5 Western Blots. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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