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You are here:Home » Molecular Biology » MB-Fluorescent Labels » 4,6-Diamidino-2-phenylindole, Dihydrochloride (DAPI, 2-(4-Amidinophenyl)-6-indolecarbamidine, Dihydrochloride)

4,6-Diamidino-2-phenylindole, Dihydrochloride (DAPI,
2-(4-Amidinophenyl)-6-indolecarbamidine, Dihydrochloride)


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A cell-permeable DNA-binding dye. Binds preferentially to DNA rich in adenine and thymine. Used in flow cytometry for measuring nuclear DNA content or for sorting isolated chromosomes.
Catalog #D3449
Melting Point>300°C
AtmosphereInert Gas
SolubilityWater, Methanol, DMSO
StabilityLight Sensitive
Method for Determining IdentityProton NMR Spectroscopic Analysis
TLC ConditionsSiO2: Dichloromethane: Methanol; Acetic Acid; 2: 2: 1; Visualized with UV and AMCS; Elongated spot; Rf=0.5-0.75.
Chloride content: 21.8%. Contains 3% methanol by NMR.
Storage and StabilityMay be stored at RT for short-term only. Long-term storage is recommended at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Toxicity and HazardsDue to the potential danger associated with the use of some of United States Biological biochemicals, all products should be handled by qualified personnel only, trained in laboratory procedures, and familiar with potential hazards. Information is not available on the possible hazards of many experimental compounds. The absence of a warning must not be interpreted as indication of safety. The ultimate responsibility for the proper handling of any and all chemicals lies with the user. All goods sold by United States Biological carry the condition that liability for any injury, loss or damage whatsoever to persons or real property resulting from the handling of the said goods lies with the user. Acceptance of our goods binds the customer to these terms.
CAS Number28718-90-3
Molecular FormulaC16H17Cl2N5
Molecular Weight350.25
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. Toxicity and Hazards: All products should be handled by qualified personnel only, trained in laboratory procedures.
Alternate namesDAPI, 2-(4-Amidinophenyl)-6-indolecarbamidine, Dihydrochloride

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