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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified B

Dicer functions is a RNase III-like enzyme which cleaves double-stranded RNA into 21-23 nt RNAs (siRNA short interfering RNA). It is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small RNA component that represses gene expression. Two transcript variants encoding the same protein have been identified.
Catalog #	D3520
Applications	Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution	Western Blot: 1:500-1:1000. Detects ~217kD band
	Optimal dilutions to be determined by the researcher.
Positive Control	Whole ovary tissue lysate
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Concentration	~0.5mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide, 0.2% gelatin, before the addition of glycerol to 40%.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Synthetic peptide (mouse), corresponding to amino acids FTKHQVLIMTCYVALTVLK (132-150) and QNKQILVPELCAIHPI (1017-1033).
Specificity	Recognizes mouse Dicer. Species sequence homology: human and rat due to over 90% sequence with immunogen, but testing shows no response in human and rat ovary at the recommended testing conditions.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.