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You are here:Home » Biochemicals » Biochem-Intermediates » 3,5-Dichloro-2-hydroxybenzenesulfonic acid, sodium salt (HDCBS-Na)

3,5-Dichloro-2-hydroxybenzenesulfonic acid, sodium salt (HDCBS-Na)


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Chromogenic reagent for HDL cholesterol. HDL (High Density Lipoproteins) are responsible for the reverse transport of cholesterol from peripheral cells to the liver, where cholesterol is transformed to bile acids. These are excreted into the intestine via the biliary tract. Monitoring of HDL-cholesterol in serum is of clinical importance because there is an inverse correlation between serum HDL-cholesterol concentrations and the risk of atherosclerotic disease. It is believed that elevated HDL-cholesterol concentrations offer protection against coronary heart disease, while reduced HDL-cholesterol concentrations, particularly in conjunction with elevated triglycerides, increase cardiovascular risk.
Catalog #D3530-84
The assay is performed in three discrete steps. In the first step, cholesterol esters are cleaved by the action of cholesterol esterase. In the second step, free cholesterol is oxidized by cholesterol oxidase to yield cholest-3-on and hydrogen peroxide. In the third step, horseradish peroxidase will catalyze the reaction of hydrogen peroxide and 4-aminoantipyrine (4-AAP) in combination with 2-hydroxy-3,5-dichloro-benzenesulfonate (HDCBS) to yield the intensely red dye quinoneimine. The level of absorbance at 520nm is proportional to the concentration of HDL cholesterol in the sample.
Solubility Clear, almost colorless and complete
(10 % in water)
Water (K. Fischer) 0.5 %
pH (0.1%, H2O) 5.0 ± 1.0
FTIR Corresponds to reference spectrum
CAS Number54970-72-8
Molecular FormulaC6H3Cl2O4SNa
Molecular Weight265.05
Purity98% (TLC, (100mg in 3ml of water; 1-butanol/acetic acid/water 3
FormWhite to almost white crystalline powder
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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