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You are here:Home » Antibodies » Antibodies-Amino Acids » Anti -4,5-Dihydroxytryptamine, Conjugated

Anti -4,5-Dihydroxytryptamine, Conjugated


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Clone Host Grade Applications
Polyclonal Rabbit Purified E
Catalog #D3680
Applications Suitable for use in ELISA. Other applications not tested.
Specificity Using a conjugate 4,5-dihydroxytryptamine-Glutaraldehyde-Protein, antibody specificity was performed with an ELISA test by competition experiments with the following compounds
Compounds Cross-reactivity ratio(a) 4,5-dihydroxytryptamine-G-BSA 1 5,6-dihydroxytryptamine-G-BSA 1/20,000 5-hydroxytryptamine-G-BSA 1/50,000
(a) 4,5-dihydroxytryptamine-G-BSA concentration/unconjugated or conjugated indolealkylamine concentration at half displacement.
G = Glutaraldehyde, BSA = Bovine Serum Albumin
Recommended dilution The anti-conjugated 4,5-dihydroxytryptamine antibodies stains well between a 1/2,000-1/5,000 dilution.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
FormSupplied as a liquid in PBS, pH 7.2. No preservative added.
PurityPurified by affinity chromatography (G200).
SpecificityRecognizes protein conjugated Dihydroxytryptamine, 4,5-. See additional crossreactivity data.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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