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You are here:Home » Antibodies » Abs to Oncoproteins » Anti -DJ-1 (PARK7, Parkinson Disease (Autosomal Recessive, Early Onset) 7, FLJ27376, Oncogene DJ1)

Anti -DJ-1 (PARK7, Parkinson Disease (Autosomal Recessive, Early Onset) 7, FLJ27376,
Oncogene DJ1)


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Clone Host Grade Applications
Monoclonal Mouse Ascites B IC
DJ-1 protein has 189 amino acids and was cloned from a HeLa cell cDNA library as a novel oncogene which weakly transformed NIH3T3 cells. Transformation by DJ-1 was enhanced by cotransfection with H-Ras or Myc. The expression of DJ-1 is induced by growth signals and it is translocated from the cytoplasm to the nucleus during the S phase, although no nuclear localization signals are present. It has been suggested that DJ-1 is a mitogen-dependent oncogene product involved in Ras-related signal transduction pathway (1). Northern blotting has shown that DJ-1 is ubiquitously expressed in various human tissues.
Catalog #D3790-01
ApplicationsSuitable for use in Western Blot and Immunocytochemistry. Other applications not tested.
Recommended DilutionImmunoblot Analysis: 1:1000-1:5000 dilution of this lot detected DJ-1 in RIPA lysates from MCF-7 and LNCaP cells. LNCaP cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-DJ-1 (1:5000 dilution). Proteins were visualized using a goat anti-mouse, IgM secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates DJ-1.
Immunocytochemistry: Has been reported by an independent laboratory to detect DJ-1 in MCF-7, LNCaP and PC-3 cells that had been permeabilized with cold methanol and fixed with formaldehyde.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS NumberN/A
Clone TypeMonoclonal
Clone No5H62
ConcentrationNot determined
FormSupplied as a liquid with 0.05% sodium azide before the addition of glycerol to 30%.
ImmunogenGST fusion protein corresponding to full length human DJ-1.
SpecificityRecognizes human DJ-1, Mr 20-25kD.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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