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You are here:Home » Antibodies » Abs to Oncoproteins » Anti -DJ-1 (PARK7, Parkinson Disease (Autosomal Recessive, Early Onset) 7, FLJ27376, Oncogene DJ1)

Anti -DJ-1 (PARK7, Parkinson Disease (Autosomal Recessive, Early Onset) 7, FLJ27376,
Oncogene DJ1)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Ascites B IC
DJ-1 protein has 189 amino acids and was cloned from a HeLa cell cDNA library as a novel oncogene which weakly transformed NIH3T3 cells. Transformation by DJ-1 was enhanced by cotransfection with H-Ras or Myc. The expression of DJ-1 is induced by growth signals and it is translocated from the cytoplasm to the nucleus during the S phase, although no nuclear localization signals are present. It has been suggested that DJ-1 is a mitogen-dependent oncogene product involved in Ras-related signal transduction pathway (1). Northern blotting has shown that DJ-1 is ubiquitously expressed in various human tissues.
Catalog #D3790-01
ApplicationsSuitable for use in Western Blot and Immunocytochemistry. Other applications not tested.
Recommended DilutionImmunoblot Analysis: 1:1000-1:5000 dilution of this lot detected DJ-1 in RIPA lysates from MCF-7 and LNCaP cells. LNCaP cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-DJ-1 (1:5000 dilution). Proteins were visualized using a goat anti-mouse, IgM secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates DJ-1.
Immunocytochemistry: Has been reported by an independent laboratory to detect DJ-1 in MCF-7, LNCaP and PC-3 cells that had been permeabilized with cold methanol and fixed with formaldehyde.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgM
Clone No5H62
HostMouse
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid with 0.05% sodium azide before the addition of glycerol to 30%.
PurityAscites
ImmunogenGST fusion protein corresponding to full length human DJ-1.
SpecificityRecognizes human DJ-1, Mr 20-25kD.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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