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You are here:Home » Antibodies » Abs to Dickkopf Proteins » Anti -Dkk-1 (DKK1, SK, DKK-1, Dickkopf Homolog 1 (Xenopus laevis), Dickkopf-1 Like, Dickkopf (Xenopus laevis) Homolog 1)

Anti -Dkk-1 (DKK1, SK, DKK-1, Dickkopf Homolog 1 (Xenopus laevis), Dickkopf-1 Like,
Dickkopf (Xenopus laevis) Homolog 1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
The human Dickkopf (Dkk)-related proteins are a structurally and functionally heterogeneous family of proteins. This family is comprised of hDkk1, -2, -3, -4 and Soggy (Sgy), a Dkk-3 related protein. Members of the Dkk-related family display unique patterns of mRNA expression in human and mouse tissues and are secreted when expressed in 293T cells. Several secreted antagonists, including Dickkopf-1 (Dkk-1) (German for “big head, stubborn”) regulate the activity of Wnt proteins secreted glycoproteins that influence many aspects of embryonic development in metazoans. Dkk-1 is a head inducer secreted from the vertebrate head organizer, that induces anterior development of Xenopus by antagonizing Wnt signaling. Dkk-1 inhibits Wnt-induced axis duplication, acts upstream of disheveled3 and antagonizes Wnt signaling upstream of its effect on beta-catenin regulation. Dkk-1 is an LRP6 ligand5 that inhibits Wnt signaling by blocking Wnt-induced Fz-LRP6 complex formation. Dkk-1 plays a crucial role in the control of programmed cell death during vertebrate limb development. Bmp-4 is one of several upstream regulators of Dkk-1 expression. Furthermore, the proapoptotic hDkk-1 gene may play important roles in linking the oncogenic Wnt and p53 tumor suppressor pathways.
Catalog #D3810
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 0.1-2ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration0.125mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide, before the addition of glycerol to 40%.
PurityPurified by epitope affinity chromatography.
ImmunogenSynthetic peptide derived from the C-terminal region of the human and mouse Dkk-1(Dickkopf-1) proteins.
SpecificityRecognizes human and mouse Dkk-1 proteins. On Western blots, it identifies the target band at ~35kD. Reactivity has been confirmed with human Dkk-1-transfected 293T cell lysates.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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