Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications Monoclonal Mouse Purified IH Gel

This clone is a reliable positive control in assays for detection of DNA antibodies, especially for the detection of autoantibodies in autoimmune diseases. It can also be used for sensitive detection of mycoplasma contamination in cell cultures.
Catalog #	D3875-01
Applications	Suitable for use in Immunohistochemistry (frozen and paraffin-embedded tissue) and Dot blot on nitrocellulose membrane (after baking at 70°C).
Recommended Dilution	Immunohistochemistry: 1:10 with PBS, pH 7.4; Incubation Time 1hr at RT.
Storage and Stability	Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgM
Clone No	5F151
Host	Mouse
Concentration	~0.1mg/ml
Form	Supplied as a lyophilized powder.
Purity	Gel filtration.
Immunogen	Double- and single-stranded DNA.
Specificity	Reactivities on cultured XLKE-A6 (Xenopus), RVF-SMC, cell lines (tested so far) PTK-2, HeLa species (tested so far). Binding characteristics: Reacts with all forms of native and denatured DNA; reactive also with lambda DNA and synthetic DNA. Antigen recognized in All DNA-containing species.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.