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You are here:Home » Antibodies » Abs to Nucleic Acids » Anti -DNA, ds

Anti -DNA, ds

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Supernatant E IC
Catalog #D3878-12
ApplicationsSuitable for use in EIA and Immunocytochemistry. Other applications not tested.
Recommended DilutionOptimal dilution determined by the researcher.
Binding AssaySupernatant was incubated with 32P-end labeled heat-denatured (ss)DNA or duplex (ds)DNA restriction fragments (20 pmoles nucleotides; 16,000 cpm) followed by precipitation of anti- DNA/DNA complexes with excess rabbit anti-mouse and quantitation of bound (32P) DNA by Chernkov radiation.
Antigen Binding ProfileNatural DNA
dsDNA(a) ssDNA(b)
1.00 0.45
Synthetic homopolymers
poly(dA) poly(dC) poly(dG) poly(dT) poly(dI) poly(dU).
0.01 0.01 0.01 0.01 0.01 0.01
Nucleic acids were complexed with solid phase mBSA and incubated with anti-DNA antibody. Radioiodinated protein A binding at the highest antibody quantity tested (either 10ug of purified antibody or a 12 culture supernatant dilution) was measured and normalized relative to that determined for the preferred antigen (given a value of 1.0). a) Native calf thymus DNA b) Heat-denatured calf thymus DNA
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2a,k
Clone No2Q2203
HostMouse
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid.
PurityTissue culture supernatant
ImmunogenDouble stranded DNA (dsDNyA) (B-form) from human
SpecificityDouble stranded DNA (dsDNyA) (B-form) from human. Crossreactivity
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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