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Specifications

Clone Host Grade Applications Monoclonal Mouse Purified E IC

Catalog #	D3878-12
Applications	Suitable for use in EIA and Immunocytochemistry. Other applications not tested.
Recommended Dilution	Optimal dilution determined by the researcher.
Binding Assay	Supernatant was incubated with 32P-end labeled heat-denatured (ss)DNA or duplex (ds)DNA restriction fragments (20 pmoles nucleotides; 16,000 cpm) followed by precipitation of anti- DNA/DNA complexes with excess rabbit anti-mouse and quantitation of bound (32P) DNA by Chernkov radiation.
Antigen Binding Profile	Natural DNA
	dsDNA(a) ssDNA(b)
	1.00 0.45
	Synthetic homopolymers
	poly(dA) poly(dC) poly(dG) poly(dT) poly(dI) poly(dU).
	0.01 0.01 0.01 0.01 0.01 0.01
Nucleic acids were complexed with solid phase mBSA and incubated with anti-DNA antibody. Radioiodinated protein A binding at the highest antibody quantity tested (either 10ug of purified antibody or a 1	2 culture supernatant dilution) was measured and normalized relative to that determined for the preferred antigen (given a value of 1.0). a) Native calf thymus DNA b) Heat-denatured calf thymus DNA
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add glycerol (40-50%) and freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2a,k
Clone No	2Q2203
Host	Mouse
Source	Human
Concentration	Not determined
Form	Supplied as a liquid.
Purity	Tissue culture supernatant
Immunogen	Double stranded DNA (dsDNyA) (B-form) from human
Specificity	Double stranded DNA (dsDNyA) (B-form) from human. Crossreactivity
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.