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You are here:Home » Kits and Assays » Kits for ELISA (AutoImmune), BioAssay™ » Anti-Double Stranded DNA (dsDNA) BioAssay™ ELISA Kit

Anti-Double Stranded DNA (dsDNA) BioAssay™ ELISA Kit

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Specifications

The United States Biological Anti-dsDNA, Human, BioAssay™ ELISA Kit is a quantitative enzyme immunoassay (EIA) intended to screen for the presence of dsDNA antibodies in sample serum as for research of systemic lupus erythematosus (SLE).
Catalog #D3878-14A
Antinuclear antibodies (ANAs) directed against a variety of macromolecules occur in extraordinarily high frequency in systemic rheumatic diseases. Many rheumatic diseases are characterized by the presence of one or more of these ANAs.
Anti-dsDNA is present in 50-70% of patients with SLE. Circulating DNA/anti-DNA immune complexes are considered to play a part in the pathogenesis of SLE. The presence of anti-dsDNA is one of the diagnostic criteria for SLE. IgG antibodies to dsDNA are considered clinically most useful for the diagnosis and management of SLE. Antibodies to single stranded DNA (ssDNA) and IgM antibodies to dsDNA are found in a number of other connective diseases, liver diseases, as well as in some normal individuals. Accurate detection of anti-dsDNA is important in the diagnosis and management of SLE. EIA tests for anti-dsDNA have demonstrated greater sensitivity than standard IFA and RIA tests allowing for improved detection of low titer antibodies to dsDNA.
Kit ComponentsEach kit contains the following components in sufficient quantities to perform the number of tests indicated on the package label.
1. Microtiter Plate: 1X96 wells
2. Wash Concentrate, 16.7X: 1X60ml
3. Sample Diluent: 4X30ml
4. IgG (HRP) Goat x human: 1X15ml
5. Positive Control: 1X450ul
6. Calibrator: 1X450ul
7. Negative Control: 1X450ul
8. Tetramethylbenzidine (TMB): 1X15ml
9. Stop Solution: 1X15ml (1N H2SO4)
Storage and StabilityStore all components at 4°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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