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Specifications

Clone Host Grade Applications Monoclonal Mouse Purified E IH IC FC IF

Anti-ssDNA (Single-stranded DNA) allows the important discrimination between apoptosis and necrosis. In this respect it can be regarded as a unique research tool superior to TUNEL staining. The specificity of the assay is based upon the high sensitivity of DNA to thermal denaturation in condensed chromatin of apoptotic cells. Although there is no change of the original product there have been important improvements in the staining protocol involving a formamide-based DNA-denaturation. This is now more convenient to use, yielding a sharper contrast between apoptotic cells and controls.
Catalog #	D3878-16B
Applications	Suitable for use in Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry and Immunofluorescence. Other applications not tested.
Recommended Dilutions	Immunohistochemistry: Frozen and paraffin sections
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgM
Clone No	6D353
Host	Mouse
Source	Bovine
Concentration	~0.1mg/ml
Form	Supplied as a liquid in PBS, 5% fetal bovine serum (FBS).
Purity	Purified
Immunogen	Calf thymus ssDNA
Specificity	Recognizes bovine deoxycytidine of ssDNA of at least 25-30 bases in length in the absence of any reactivity to double-stranded DNA. Not species-specific. Specifically detects apoptotic cells. Necrotic cells are not recognized.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.